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Method for extracting and separating astragalin from plant Mesona chinensis Benth

A technology of astragalus glycosides and celestial grass, which is applied in the direction of chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of waste of resources, etc., and achieve simple and easy process, cheap and easy-to-obtain raw materials, and high purity Effect

Inactive Publication Date: 2010-12-22
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And some substances with poor water solubility are discarded as waste, resulting in a huge waste of resources
At present, there is no literature report on the extraction of astragalus glycosides from celestial grass

Method used

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  • Method for extracting and separating astragalin from plant Mesona chinensis Benth
  • Method for extracting and separating astragalin from plant Mesona chinensis Benth
  • Method for extracting and separating astragalin from plant Mesona chinensis Benth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] ① Take 10g of the cut, ground and sieved whole grass jelly, add about 100mL of 75% acetone solution, extract at 60°C, extract for 2 hours, extract twice, filter while hot, combine the filtrate, concentrate under reduced pressure to remove the solvent, and dry in vacuum The crude extract was obtained, and its polyphenol content reached 205.1±7.1 mg GAE / g extract, and the specific data are shown in Table 1.

[0024] Table 1 Example 1 rough extraction data

[0025]

[0026] ② Crude extract 1g, separated by AB-8 weak polar macroporous adsorption column chromatography (mobile phase is ethanol water solution with different volume fractions: first remove impurities with water, then elute with 60% ethanol, and finally elute with 95% ethanol Residue in the column), the 60% ethanol eluted fraction was collected, concentrated under reduced pressure to remove the solvent, and dried in vacuo to obtain the refined extract with a polyphenol concentration of 534.9 mg GAE / g. The spec...

Embodiment 2

[0031] ① Take 10g of the cut, ground and sieved whole grass jelly, add about 100mL of 75% acetone solution, extract at 60°C, extract for 2 hours, extract twice, filter while hot, combine the filtrate, concentrate under reduced pressure to remove the solvent, and dry in vacuum The crude extract was obtained, and its polyphenol content reached 205.1±7.1 mg GAE / g extract, and the specific data are shown in Table 1.

[0032] ② Crude extract 1g, separated by DM130 weak polarity macroporous adsorption column chromatography (mobile phase is ethanol aqueous solution with different volume fractions: first remove impurities with water, then elute with 60% ethanol, and finally elute the column with 95% ethanol residue), collected 60% ethanol eluted fractions, concentrated under reduced pressure to remove the solvent, and dried in vacuo to obtain the refined extract with a polyphenol concentration of 298.5 mg GAE / g. The specific data are shown in Table 3.

[0033] Table 3 Example 2 refine...

Embodiment 3

[0037] ① Take 10g of the cut, ground and sieved whole grass jelly, add about 100mL of 75% acetone solution, extract at 60°C, extract for 2 hours, extract twice, filter while hot, combine the filtrate, concentrate under reduced pressure to remove the solvent, and dry in vacuum The crude extract was obtained, and its polyphenol content reached 205.1±7.1 mg GAE / g extract, and the specific data are shown in Table 1.

[0038] ② Crude extract 1g, separated by HPD450 weak polar macroporous adsorption column chromatography (mobile phase is ethanol aqueous solution with different volume fractions: first remove impurities with water, then elute with 60% ethanol, and finally elute the column with 95% ethanol residue), collected 60% ethanol eluted fractions, concentrated under reduced pressure to remove the solvent, and vacuum dried to obtain the refined extract, whose polyphenol concentration was 415.9 mg GAE / g, and the specific data are shown in Table 4;

[0039] Table 4 Example 3 refin...

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Abstract

The invention provides a method for extracting and separating astragalin from the plant Mesona chinensis Benth. The method comprises the following steps: using the water-acetone solution for Mesona chinensis Benth for extraction, concentrating and drying the extract to obtain a crude extract, performing chromatographic separation to the crude extract with a macroporous adsorption resin column, collecting eluent, concentrating and drying the eluent to obtain a fine extract, processing the fine extract through high performance liquid chromatography to separate and prepare an astragalin pure product with the purity of 99%. The raw material of the invention is cheap and accessible and barely causes environmental pollution; the technology is simple, practical and fast; and the obtained astragalin has high purity and suitable for mass industrial production.

Description

technical field [0001] The invention relates to a method for extracting and isolating astragalus glycosides from plant jelly. Background technique [0002] Grass jelly, also known as Xianrenliang, jelly grass, and firewood, is an annual herb of the family Lamiaceae, and is an important oriental plant with both medicinal and edible uses. Grass jelly has high medicinal value. Traditional Chinese medicine believes that grass jelly is cool and cold in nature, sweet and light in taste, and has the effects of clearing heat and dampness, cooling blood and relieving heat, quenching thirst and diuresis, and removing heat and toxins. Among the functional ingredients contained in Grass jelly, polysaccharides can enhance human immunity, and some have antioxidant properties; polyphenols can scavenge free radicals and have antioxidant properties; trace elements can inhibit the formation of free radicals, anti-aging, anti-aging cancer; ursolic acid and oleanolic acid can lower blood press...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
Inventor 王碧玉邱挺吴燕翔郑辉东林晓翠李玲
Owner FUZHOU UNIVERSITY
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