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Method for improving enzyme production and secondary metabolites of fungi

A technology for secondary metabolites and secondary metabolites, applied in the fields of medicine, chemical industry, and microorganisms, to achieve the effect of increasing concentration, increasing efficiency, and improving impact

Inactive Publication Date: 2012-07-04
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these means can effectively reduce the formation of shear stress, they still have relatively large limitations for the production of enzymes and secondary metabolites in some fungi, and the effect is not very satisfactory. Further improvement and improvement are needed.

Method used

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  • Method for improving enzyme production and secondary metabolites of fungi
  • Method for improving enzyme production and secondary metabolites of fungi
  • Method for improving enzyme production and secondary metabolites of fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The production of embodiment 1 lignin hydrolase

[0031] (1) Trametes versicolor (Trametes versicolor) strain is used, and its number is: China Center for Type Culture Collection (CCTCC) 5.48, which can be provided by strain preservation institutions such as China Center for Microorganism Collection;

[0032] (2) Put 1ml of the spore suspension of Trametes versicolor (Trametes versicolor) strain into potato / glucose medium, and cultivate it at 30°C and 150-200rpm for 24-28 hours to obtain the strain culture solution;

[0033] (3) Composition of fermentation medium:

[0034] Potassium dihydrogen phosphate 2.0g / L,

[0035] Ammonium sulfate (NH4) 2 SO 4 1.4g / L,

[0036] CaCl 2 2H 2 O 0.1g / L,

[0037] MgSO 4 ·7H 2 O 0.5g / L,

[0038] Glucose 10g / L,

[0039] Trace element solution 1-10ml,

[0040] Vitamin B1 0.001g / L,

[0041] Diammonium tartrate 0.02g / L,

[0042] Poloxamer (Pluronic) F68 0-3g / L;

[0043] (4) Fermentation conditions: Inoculate the strain cul...

Embodiment 2

[0051] The production of embodiment 2 cellulolytic enzymes

[0052] (1) Trichoderma viride (Trichoderma viride) bacterial strain is used, and its serial number is: 3.1912, which can be provided by bacterial strain preservation institutions such as China Center for Type Culture Collection (CCTCC);

[0053] (2) Insert 1 ml of the spore suspension of Trichoderma viride (Trichoderma viride) bacterial strain into the seed culture medium, and cultivate at 150-200 rpm for 16-24 hours at 30°C;

[0054] (3) Composition of fermentation medium:

[0055] Inorganic nutrient solution:

[0056] K H 2 PO 4 2.0g / L,

[0057] Urea 0.3g / L,

[0058] (NH4) 2 SO 4 1.4g / L,

[0059] CaCl 2 0.3g / L,

[0060] MgSO 4 ·7H 2 O 0.3g / L,

[0061] FeSO 4 ·7H 2 O 0.005g / L,

[0062] MnSO 4 ·H 2 O 0.0016g / L,

[0063] ZnSO 4 0.0014g / L,

[0064] CoCl 2 0.002g / L;

[0065] On the basis of inorganic nutrient solution, add:

[0066] Lactose 10g / L,

[00...

Embodiment 3

[0072] The production of embodiment 3 xylanase

[0073] (1) Aspergillus niger (Aspergillus niger) strain is used, its number is: 3.3147, which can be provided by strain preservation institutions such as China Center for Type Culture Collection (CCTCC);

[0074] (2) Inject 1 ml of the spore suspension of Aspergillus niger into the potato / glucose seed culture medium, and cultivate at 150-200 rpm for 16-24 hours at 30°C;

[0075] (3) Composition of fermentation medium:

[0076] Inorganic nutrient solution:

[0077] K H 2 PO 4 2.0g / L,

[0078] (NH4) 2 SO 4 1.4g / L,

[0079] CaCl 2 0.3g / L,

[0080] MgSO 4 ·7H 2 O 0.3g / L,

[0081] FeSO 4 ·7H 2 O 0.005g / L,

[0082] MnSO 4 ·H 2 O 0.0016g / L,

[0083] ZnSO 4 0.0014g / L,

[0084] CoCl 2 0.002g / L;

[0085] Fermentation medium: based on inorganic nutrient solution, adding

[0086] Peptone 1g / L,

[0087] Lactose 10g / L,

[0088] Pluronic F68 1g / L;

[0089] (4...

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Abstract

The invention relates to a method utilizing fungi to produce secondary metabolites, protein and other bioactivators. In the method, spore solution preparation, seed culture, fermentation production, extraction separation and other steps are carried out to obtain enzyme and secondary metabolites; the adopted strains are Aspergillus terreus, Aspergillus niger, Trametes versicolor and Trichoderma viride; and during fermentation production, non-ionic surfactant poloxamer F68 is added to serve as shear force protective agent to produce enzyme or other metabolites. The invention has the positive effects of improving the influence of shear force generated from stirring and air introduction on metabolism and product synthesis and improving the efficiency of producing enzyme and secondary metabolites by fungi fermentation in industrial production through adding the shear force protective agent in a fermentation medium.

Description

【Technical field】 [0001] The invention relates to the technical fields of microorganisms, medicine and chemical industry, in particular to a method for producing secondary metabolites, proteins and other bioactive substances by using fungi. 【Background technique】 [0002] Filamentous fungi, as producers of various products, are widely used in industrial production, especially in the production of secondary metabolites, proteins and other biologically active substances, such as antibiotics, polysaccharides, and enzyme preparations. The production process using filamentous fungi includes both solid and semi-solid fermentation processes and submerged liquid fermentation processes. The first two processes can directly use low-value raw materials, have less water and energy input, and are simple to operate, but the production efficiency is low, making it difficult to carry out large-scale and stable production; in addition, the use of solid-state fermentation process, the transf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N9/24C12P17/06C12P1/02C12R1/645C12R1/66C12R1/885C12R1/685
Inventor 李志敏祖彩霞叶勤
Owner EAST CHINA UNIV OF SCI & TECH
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