Granular type compound microbial bacterial agent as well as preparation method and application thereof
A composite microbial inoculum, granular technology, applied in the application, fertilizer mixture, organic fertilizer and other directions, can solve the problems of low number of viable bacteria and large differences in water content after adsorption, to reduce and absorb residual pesticides and Effects of toxic substances, increased fertilizer intensity, and improved utilization
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Embodiment 1
[0051] 1. Strain fermentation
[0052] Bacillus subtilis (Bacillus subtilis), Bacillus megaterium (Bacillus megaterium), jelly-like Bacillus (Bacillus mucilaginosus), three strains were respectively subjected to slant culture, shaker expansion culture, seed tank fermentation and fermenter fermentation, After fermentation, the number of viable bacteria in the bacterial liquid reaches 3.5-4.5 billion / ml; (for strain fermentation, please refer to figure 1 )
[0053] 2. Bacterial liquid adsorption: the fermented bacterial liquid of a single strain is adsorbed with medical stone respectively in the adsorption stirring equipment, (the adsorption ratio is by Bacillus subtilis bacterial liquid: medical stone=1:5 (L / kg) , the adsorption ratio is by bacillus megaterium bacterium liquid: medical stone=1: 5 (L / kg), and the adsorption ratio is by jelly-like bacillus: medical stone=1: 5 (L / kg)), then stir Evenly, then mix and mix according to the ratio of 2:2:1; (for the preparation of po...
Embodiment 2
[0070] In the preparation of powdery composite microbial bacterial agent: the fermented bacterium liquid of single strain is adsorbed with medical stone respectively in the adsorption stirring equipment, (adsorption ratio is by Bacillus subtilis bacterium liquid: medical stone=1: 4( L / kg), the adsorption ratio is according to bacillus megaterium bacteria liquid: medical stone=1: 6 (L / kg), and the adsorption ratio is according to jelly-like bacillus: medical stone=1: 5 (L / kg) ), then stir evenly, then mix and mix according to the ratio of 2:3:1. In the production ratio of the composite microbial bacterial agent, the ratio between them is powdery composite microbial bacterial agent: phosphate rock powder: medium and trace elements=80:14:6. Others are with embodiment 1.
Embodiment 3
[0072] In the preparation of powdery composite microbial bacterial agent: the fermented bacterium liquid of single bacterial species is adsorbed with medical stone respectively in the adsorption stirring equipment, (adsorption ratio is by Bacillus subtilis bacterium liquid: medical stone=1: 6( L / kg), the adsorption ratio is according to bacillus megaterium bacteria liquid: medical stone=1: 6 (L / kg), and the adsorption ratio is according to jelly-like bacillus: medical stone=1: 5 (L / kg) ), then stir evenly, then mix and mix according to the ratio of 1:1:1. In the production ratio of the composite microbial bacterial agent, the ratio between them is powdery composite microbial bacterial agent: phosphate rock powder: medium and trace elements=90:8:2. Others are with embodiment 1.
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