Method for ultrasonic extraction of total saponins of rhizoma panacis majoris

A technology of pearl ginseng and total saponins, which can be used in pharmaceutical formulas, medical preparations containing active ingredients, anti-toxins, etc., and can solve problems such as the best ultrasonic extraction process that has not yet been seen.

Inactive Publication Date: 2011-01-19
SHAANXI NORMAL UNIV
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  • Method for ultrasonic extraction of total saponins of rhizoma panacis majoris
  • Method for ultrasonic extraction of total saponins of rhizoma panacis majoris
  • Method for ultrasonic extraction of total saponins of rhizoma panacis majoris

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[0020] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0021] 1.2 Experimental part

[0022] 1.2.1 Instruments, reagents and materials

[0023] Instruments: UV-1600 ultraviolet spectrophotometer (Beijing Rayleigh Analytical Instrument Co., Ltd.), SL202N pharmaceutical electronic balance (Shanghai Mingqiao Precision Scientific Instrument Co., Ltd.), RE-52AA rotary volatile instrument (Shanghai Yarong Biochemical Instrument Factory), glass Instrument airflow dryer (Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.), HH-6B digital display constant temperature water bath (Guohua Electric Co., Ltd.), KQ-100E ultrasonic cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd.), medicinal plant crushing Machine (Huanghua Zhongxing Instrument Co., Ltd.).

[0024] Reagents: Ginsenoside Re reference substance (National Institute for the Control of Pharmaceutical and Biological Products), vani...

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Abstract

The invention discloses a method for the ultrasonic extraction of the total saponins of rhizoma panacis majoris, which comprises: drying rhizoma panacis majoris, crushing and sieving with a 40-mesh sieve, weighing coarse powder, placing in a Soxhlet extractor, adding petroleum ether, refluxing for 2 hours, taking a filter paper barrel out and drying medicinal materials in the air for later use; and weighing processed materials, adding 30 to 70 percent ethanol into the materials according to a material-to-liquid ratio (g/mL) of 1:10-20, fully and uniformly shaking, soaking for 1 hour, and ultrasonically extracting for 10 to 30 minutes at 30 to 50 DEG C. The operation of the ultrasonic extraction method has the advantages of simplicity, time conservation, safety, convenience, low cost, high extraction rate, complete components and the like.

Description

technical field [0001] The invention relates to the technical field of extracting effective components of Chinese herbal medicines, in particular to a method for ultrasonically extracting total saponins from ginseng. Background technique [0002] Saponins, also known as alkali soap body, saponin, saponin, saponin or saponin, is a kind of glycoside compound with complex structure existing in the plant kingdom, which can form aqueous solution or colloidal solution and can form soap The phytoglycosides of the foam are collectively referred to. According to the molecular structure of known saponins, saponins can be divided into two categories, one is steroidal saponins, and the other is triterpene saponins. Saponins are mostly white or milky white amorphous powder, a few are crystalline, bitter and pungent, and irritating to mucous membranes. Saponins are generally soluble in water, methanol and dilute ethanol, easily soluble in hot water, hot methanol and hot ethanol, insolub...

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Application Information

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IPC IPC(8): A61K36/25A61K36/34A61P1/04A61P9/10A61P25/04A61P25/20A61P39/00A61K125/00
Inventor 张延妮岳宣峰王喆之
Owner SHAANXI NORMAL UNIV
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