Specific molecular markers of sex chromosomes of Tilapia nilotica and genetic sex identification method

Abstract

The invention belongs to the technical field of genetic engineering, in particular to specific molecular markers of sex chromosomes of Tilapia nilotica, wherein the nucleotide sequences of specific molecular markers NtY1 and NtY2 of a Y chromosome are respectively shown in SEQID No.1 and SEQID No.2, and the nucleotide sequence of a specific molecular marker NtX1 of a X chromosome is shown in SEQID No.3; in the invention, a genetic sex identification method of Tilapia nilotica is established by using the specific molecular markers of the sex chromosomes of the Nile tilapia, and through carrying out PCR amplification on any one or more than one of the specific molecular markers of the sex chromosomes, YY super-male fishes, XY milter and XX female fishes can be identified economically, quickly and accurately, thus being beneficial to realizing the large-scale production of holandric Tilapia nilotica fries through YY super-male fish breeding routes, obviously improving the breeding production of Tilapia nilotica, reducing the breeding cost, and improving the economic benefit.

Description

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AI Technical Summary

Benefits of technology

The technical effects are improved methods by combining DNA polymerase chain reaction with other techniques like restriction analysis or hybridization probing to identify individuals from yellow tiger fish populations. These new technologies have several benefits such as high accuracy, ease of use, quick response times, lower costs compared to traditional approaches, making it possible to produce more pigmentary bodies than previously available at an affordable price.

Problems solved by technology

This patents discusses different ways to identify pigmentary fish like blue turtles without being threatened due to predators during commercial harvesting. Current approaches involve sorting up entire populations of fish into smaller groups called subgroups, identifying each type separately, and performing experiments at an expense compared to other types. These current technologies require expensive equipment and time consumption, making it difficult to achieve accurate separation between nonbred and wild ones while also maintaining desired fertility levels throughout generations.

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