Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting lung cancer susceptibility by MAP2K4 gene and application thereof

A gene detection and kit technology, applied in the field of medical research, can solve the problem of reducing the incidence of tumors and achieve the effect of wide application

Inactive Publication Date: 2011-02-16
广州医学院
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor's research also shows that rs3826392 is related to the occurrence of sporadic intestinal cancer and nasopharyngeal carcinoma, both of which show that the incidence of tumors is reduced when the rs3826392 genotype is G

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting lung cancer susceptibility by MAP2K4 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0028] This example includes:

[0029] 1) The materials of the kit include: a pair of specific primers and a pair of specific fluorescent probes for detecting the SNP site rs3826392 on the MAP2K4 gene, Taq DNA polymerase, dNTP mixture, MgCl2 solution, and fluorescent quantitative PCR reaction buffer and deionized water;

[0030] 2) The sequence of the specific primer pair for detecting the rs3826392 SNP site on the MAP2K4 gene is shown in the sequence table SEQ ID No.: 1, the Tm value of the sense primer sequence is 60°C, and the Tm value of the antisense primer sequence 58°C; the sequence of the specific fluorescent probe pair for detecting the rs3826392 SNP site on the MAP2K4 gene is shown in the sequence table SEQ ID No.: 2, the Tm value of the wild-type probe sequence is 66°C, and the mutant type The Tm value of the probe sequence is 67°C;

[0031] 3) The specific primers can specifically amplify a pair of DNA primers and a pair of DNA probes against the SNP site rs38263...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting lung cancer susceptibility by MAP2K4 gene and application thereof. The kit contains the following substances: specific primer pair and specific fluorescent probe pair which are used for detecting SNP site rs3826392 on MAP2K4 gene, Taq DNA polymerase, dNTP mixed solution, MgCl2 solution, fluorescent quantitative PCR reaction buffer solution and deionized water as well as specific primer; wherein the sequence of specific primer pair used for detecting SNP site rs3826392 on MAP2K gene contains a sense primer sequence and an antisense primer sequence; the specific fluorescent probe pair used for detecting SNP site rs3826392 on MAP2K4 contains a wild type probe sequence and a mutant type probe sequence; and the specific primer can specifically amplify DNA primer pair and DNA probe pair which direct at the SNP site rs3826392 on MAP2K4 gene. The invention detects lung cancer susceptibility by detecting SNP site rs3826392 on MAP2K4 gene and can be widely applied to medical research.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility and its application, in particular to a kit for detecting susceptibility to lung cancer by MAP2K4 gene and its application. Acid polymorphism (SNP) loci to predict susceptibility to lung cancer. In the field of medical research. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the malignant tumors with the highest morbidity and mortality in the world. Because lung cancer often accompanies early metastasis, 70% of lung cancer patients are found at advanced stage, and the current average 5-year survival rate is only 16%. The speed of disease progression is related to the biological characteristics of lung cancer cells. Lung cancer is a disease that seriously threatens human health and life. In 2009, the United States reported 219,440 new cases of lung cancer and 159,390 deaths (mortality rate: 53.80 / 100,000). According to the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68G01N21/64
Inventor 吕嘉春刘斌
Owner 广州医学院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com