Kit for detecting acute myeloid leukemia susceptibility

An acute myeloid and kit technology, applied in the field of kits for detecting the susceptibility of acute myeloid leukemia, can solve the problem of not predicting acute myeloid leukemia, and achieve the effect of wide application

Inactive Publication Date: 2011-08-10
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no research report on the correlation between the T>G polymorphism in the promoter region of the CD44 gene and the susceptibility to acute myeloid leukemia, and there is no single nucleotide detection through the detection of mitogen-activated protein kinase kinase 4 (CD44). Acid polymorphism (SNP) loci to predict susceptibility to acute myeloid leukemia

Method used

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  • Kit for detecting acute myeloid leukemia susceptibility

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kit for detecting susceptibility to acute myeloid leukemia, comprising: a pair of specific primers and a pair of specific fluorescent probes for detecting the SNP site rs11821102 on the CD44 gene, Taq DNA polymerase, dNTP mixture, MgCl2 solution, Fluorescent quantitative PCR reaction buffer and deionized water;

[0021] The sequence of the specific primer pair for detecting the rs11821102 SNP site on the CD44 gene is shown in the sequence table SEQ ID NO: 1, the Tm value of the sense primer sequence is 60 ° C, and the Tm value of the antisense primer sequence is 58 ℃; the sequence of the specific fluorescent probe pair for detecting the rs11821102 SNP site on the CD44 gene is shown in the sequence table SEQ ID NO: 2, the Tm value of the wild-type probe sequence is 66 ℃, and the mutant-type probe sequence The Tm value is 67 ℃;

[0022] The specific primer can specifically amplify a pair of DNA primers and a pair of DNA probes against the SNP site rs11821102 on the CD4...

Embodiment 2

[0028] Step 1: Extraction of DNA

[0029] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. 5ml of EDTA anticoagulated blood was extracted by conventional method. Genomic DNA of blood samples was extracted by silica gel adsorption method.

[0030] Step 2: Genotyping Assays

[0031] Using the kit described in Example 1, perform fluorescence quantitative PCR detection on the SNP site rs11821102 on the CD44 gene of the DNA of the subject to be tested, and determine the genotype of the site.

[0032] Step 3: Instruct people to proactively prevent acute myeloid leukemia

[0033] Through the analysis of the genotype of the tested person, the genotyping test report and the individualized health guidance report for the tested person are issued. The genotyping test report details the susceptibility and probability of acute myeloid leukemia of the tested person. The individualiz...

Embodiment 3

[0035] After genotyping 314 patients with acute myeloid leukemia and 615 normal controls by the method we designed, the obtained data are drawn in Table 1:

[0036] Table 1. CD44 gene rs11821102 single nucleotide polymorphism and risk of breast cancer

[0037]

[0038] It can be seen from Table 1 that individuals carrying the CD44 rs11821102 AA genotype have a 3.05-fold higher risk of developing acute myeloid leukemia than individuals carrying the CD44 rs11821102 GG genotype, and individuals carrying the CD44 rs11821102 AG genotype The risk of developing acute myeloid leukemia was 1.68 times higher (the differences were all significant in the trend test, P < 0.05).

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Abstract

The invention relates to a kit for detecting disease susceptibility. The susceptibility of acute myeloid leukemia is predicted by detecting a single nucleotide polymorphism (SNP) site of mitogen activated protein kinase 4 (CD44). The kit comprises a fluorescence quantitative polymerase chain reaction (PCR) conventional component, a specific primer pair for detecting the No.rs11821102 SNP site of the CD44 and a specific fluorescence probe pair. The kit can detect the gene type of the No.rs11821102 SNP site on the CD44 gene of DNA so as to detect the susceptibility of individual acute myeloid leukemia, is also suitable for prognostic detection of gastric cancer, prostatic cancer and ovarian cancer, and can be widely applied in medicinal research.

Description

technical field [0001] The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting susceptibility to acute myeloid leukemia, by detecting the single nucleotide polymorphism of mitogen-activated protein kinase kinase 4 (CD44) (SNP) loci to predict susceptibility to acute myeloid leukemia. Background technique [0002] The number of cases of hematological malignancies worldwide is about 400,000, accounting for the sixth place among all kinds of malignancies. The annual new cases in China are about 250,000, accounting for the eighth place among malignant tumors in my country. The occurrence of cancer is a multi-factor, multi-stage, multi-gene mutation process. As a key molecule of cell surface function, CD44 is involved in various important processes of tumorigenesis and development. A large number of studies have shown that the single nucleotide polymorphisms of genes related to tumor immune regulation can affect the role of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 周翊峰郑健姜岚游永鹤
Owner SUZHOU UNIV
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