Blocking buffer for encapsulated plate

A technology of sealing liquid and coating plate, which can be used in measurement devices, instruments, scientific instruments, etc., can solve problems such as easy loss of activity, and achieve the effects of being non-toxic and harmless to human body, not affecting sensitivity, and simple operation.

Inactive Publication Date: 2011-03-30
刘萍 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention proposes a coated plate sealing solution to solve the problem in th

Method used

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  • Blocking buffer for encapsulated plate
  • Blocking buffer for encapsulated plate
  • Blocking buffer for encapsulated plate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The TSH chemiluminescence immunoassay method established with the anti-thyroid-stimulating hormone (TSH) monoclonal antibody coated with the embodiment of the present invention comprises the following steps:

[0021] Step 1. Prepare the coated plate blocking solution according to the above preparation method, and its components and concentrations are:

[0022] 0.02mol / L phosphate (PBS) buffer system pH7.4

[0023] Bovine Serum Albumin (BSA) 0.5g / L

[0024] Glucose 0.5g / L

[0025] Tyrosine 0.5g / L

[0026] Tween-20 (Tween-20) 0.2g / L

[0027] Gentamicin sulfate 500,000 units / L

[0028] Proclin 300 0.1g / L

[0029] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat the above-mentioned blocking solution at 200 / well at 4°C for 18 After one hour, discard the blocking solution in the coated plate, pat it dry, and dry it in a 37°C drying o...

Embodiment 2

[0037] The TSH chemiluminescence immunoassay method established with the anti-TSH monoclonal antibody coated with the embodiment of the present invention comprises the following steps:

[0038] Step 1. Prepare the coated plate blocking solution according to the above preparation method, and its components and concentrations are:

[0039] 0.02mol / L PBS buffer system pH7.4

[0040] Casein 3.5g / L

[0041] Trehalose 2.5g / L

[0042] Proline 2.5g / L

[0043] Tween-80 (Tween-80) 1.0g / L

[0044] Gentamicin sulfate 1.6 million units / L

[0045] Proclin 300 1g / L

[0046] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat the above-mentioned blocking solution at 200 / well at 4°C for 18 After one hour, discard the blocking solution in the coated plate, pat it dry, and dry it in a 37°C drying oven with a humidity of 25% for 4 hours; then place multiple...

Embodiment 3

[0054] The TSH chemiluminescence immunoassay method established with the anti-TSH monoclonal antibody coated with the embodiment of the present invention comprises the following steps:

[0055] Step 1. Prepare the coated plate blocking solution according to the above preparation method, and its components and concentrations are:

[0056] 0.02mol / L PBS buffer system pH7.4

[0057] Bovine serum albumin 10g / L

[0058] Lactose 5g / L

[0059] Glycine 10g / L

[0060] Triton X-100 (Triton X-100) 2g / L

[0061] Gentamicin sulfate 5 million units / L

[0062] Proclin 300 2g / L

[0063] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat the above-mentioned blocking solution at 200 / well at 4°C for 18 After one hour, discard the blocking solution in the coated plate, pat it dry, and dry it in a 37°C drying oven with a humidity of 25% for 4 hours; then plac...

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Abstract

The invention provides a blocking buffer for encapsulated plate, which consists of 0.5 to 10g/L of protein substances, 0.5 to 5g/L of sugar substances, 0.5 to 10g/L of amino acid substances, 0.2 to 2g/L of surfactant, 50 to 5 million unit/L of gentamicin sulphate, 0.2 to 2g/L of Proclin 300 and 0.02mol/L of phosphate buffer with the pH of 7.4. The encapsulated plate using the blocking buffer of the invention can not only block off biological active substances, but can also maintain the activity of the biological active substances for a longer time when the encapsulated plate departs from low temperature environment, thus the problem in relevant technology that the encapsulated plate is liable to lose activity when departing from lower temperature storage environment can be settled. In addition, the blocking buffer of the invention is easy in formulation, free from the impact on sensitivity, low in cost and simple in operation and has no environmental pollution and no toxicity and harm to human body.

Description

technical field [0001] The invention relates to a coated plate sealing solution. Background technique [0002] At present, the immunoassay methods used in the clinical field mainly include turbidimetric assay, immunochromatography, enzyme-linked diagnostic reagent (EIA), and chemiluminescence immunoassay (CLIA), which has been developed and applied in recent years. Among them, since trace amounts of biologically active substances (such as antigens or antibodies) are easily lost after being adsorbed onto the coated plate, the plate EIA and CLIA methods both involve a key technology, that is, to maintain the stability of the coated plate. [0003] Currently commonly used sealing solutions for coated plates can only seal the coated plates, but cannot effectively maintain the activity of biologically active substances thereon. The method to maintain the activity of biologically active substances on the coated plate is usually to store the coated plate in an environment below -2...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/577
Inventor 刘萍栾大伟
Owner 刘萍
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