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Composite adjuvant for duck bird flu oral mucosal immunization

An avian influenza and mucosal technology, which is applied to antiviral agents, medical preparations containing active ingredients, and antibody medical ingredients, etc. The effect of virus excretion polluting the environment, resisting virus infection and preventing infection

Active Publication Date: 2011-04-06
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main method currently adopted in my country is the intramuscular injection of whole-virus inactivated vaccine with oil-emulsion adjuvant, but this immunization method has the following disadvantages: waste of manpower and material resources, stress response during injection affects animal growth, and residual effects of oil-emulsion adjuvant Meat quality, etc. In addition, the special structure of waterfowl such as ducks (duck feathers are slippery and duck down is dense) also brings greater difficulties to intramuscular injection

Method used

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  • Composite adjuvant for duck bird flu oral mucosal immunization
  • Composite adjuvant for duck bird flu oral mucosal immunization
  • Composite adjuvant for duck bird flu oral mucosal immunization

Examples

Experimental program
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Embodiment Construction

[0044] Part 1: Preliminary Tests

[0045] 1 High concentration of glucose promotes the transport of fluorescently labeled molecules

[0046] Apply human colon adenocarcinoma cell monolayer (Caco-2 cells, purchased from Shanghai Chinese Academy of Sciences Cell Bank) and fluorescent permeable marker molecules (purchased from Sigma) FITC (MW 389.4), FD20S (MW 70,000) and FD2000S (MW 2,000,000) As a paracellular transport test, due to its hydrophilic properties, it can only be transported exclusively from the paracellular transport, as a test to confirm that high concentration of glucose promotes absorption. After adding glucose (750mmol / L) for 2 hours, it was found that high-concentration glucose (750mmol / L) significantly increased the translocation of FD2000S (Pfigure 1 ).

[0047] 2 High concentration of glucose promotes the absorption of collagenase in the small intestinal epithelium of mice

[0048] High-concentration glucose (1500mmol / L) and FITC-labeled collagenase (0.25...

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Abstract

The invention discloses a mucosal immunization composite adjuvant relating to an oral inactivated vaccine and belonging to the technical field of biology. The composite adjuvant is specially used for the application of the oral inactivated vaccine. The formula of the composite adjuvant comprises 20-50mug of CpG DNA (dinucleotide), 2-5mg of Astragalus mongholicus polysaccharide and 70-100mg of glucose. The composite mucosal immunization adjuvant can effectively improve the mucosal local immunization and the general humoral immunity. Meanwhile, the composite adjuvant is matched with a bird flu inactivated antigen, and therefore, an animal organism can be stimulated to generate effective general immune response level, the duck stress reaction caused by intramuscular injection is avoided and the duck quality is also ensured through drinking water immunization.

Description

1. Technical field [0001] The invention relates to a compound adjuvant for oral mucosal immunity of duck avian influenza and belongs to the field of biotechnology. It is specially designed to improve the mucosal immune effect of inactivated vaccines. 2. Background technology [0002] 1Characteristics of gastrointestinal mucosal immunity [0003] The mucosal immune system refers to the immunity on the mucosal surface of the cavity (including the respiratory tract, digestive tract and genitourinary tract) that the body communicates with the outside world. The system covers a large area in the body (more than 400m 2 ), forming the first barrier for animals to defend against external pathogens, so the mucosal immune system occupies a very important position in the body's immune system. Gastrointestinal lymphoid tissue is also known as gut-associated lymphoid tissue (GALT). Secretory IgA is the main effector of mucosal immunity. It not only has the functions of neutralizing v...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/145A61P31/16
Inventor 杨倩王红丽康海泓庾庆华
Owner NANJING AGRICULTURAL UNIVERSITY
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