Extraction method of monosialotetrahexosylganglioside

A technology of ganglioside and monosialic acid, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of burden on patients and their families, endangering patients' lives, and lack of specific drugs.

Inactive Publication Date: 2011-04-20
胡增荣 +1
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Problems solved by technology

It not only endangers the life of the patient, but more seriously, the neurological function after injury cannot be recovered well, which often brings a heavy burden to the patient and their family members. Currently, in addition to giving symptomatic supportive treatment in the acute phase, neurotrophic drugs and functional Outside of exercise, there are few specific medicines

Method used

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  • Extraction method of monosialotetrahexosylganglioside

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Experimental program
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Embodiment 1

[0023] 1. Extraction of total lipid

[0024] a. Weigh 5kg of pig brain, add 8 times the amount (40000ml) of chloroform-methanol mixture (1:3), use a high-speed tissue grinder to extract by electric stirring for 1 hour (speed 600 rpm), and let stand for 3 hours , and finally extract the supernatant, beat repeatedly three times, and combine 110L of the supernatant extracted three times.

[0025] b. Add 2500ml of water to every 10L of supernatant, stir for 1 hour, let it stand for 36 hours, and separate layers; separate the bottom chloroform layer to a total of 26L, recover after distillation and apply mechanically.

[0026] c. Separate 106 L of the supernatant aqueous phase, add 0.3% NaOH for hydrolysis, the pH is about 10, heat the water bath to boiling at 55°C±5°C for 40 minutes for hydrolysis.

[0027] d. After hydrolysis, it is detected by resorcinol, and it is purple, and the OD value is 0.2-0.35. It is cooled for later use.

[0028] 2. Coarse-porous resin exchange column...

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Abstract

The invention discloses an extraction method of monosialotetrahexosylganglioside. The extraction method mainly comprises the following steps: separating G1S in the total extract of a brain tissue total extract by adopting a macroporous resin for separating a hydrophilic and lipophilic natural product through an adsorptive process, wherein the hydrophilic and lipophilic natural product is sugar and the like; and separating out high-quality monosialotetrahexosylganglioside (GM1) dry power by common silicagel column chromatography. As different metal ionic impurities still exist in the product subject to the silicagel column chromatography, a cation exchange column is used for further ionic exchange. After improving the traditional method, a large amount of solvent and time are saved, the yield is improved, and the cost is lowered to a great extent.

Description

technical field [0001] The invention relates to a preparation method of medicine, in particular to a method for extracting monosialotetrahexosyl ganglioside from pig brain, cow brain and buffalo brain as extraction raw materials. Background technique [0002] Monosialotetrahexosyl ganglioside can promote the functional recovery of central nervous system damage caused by various reasons. The mechanism of action is to promote "neural remodeling" (including the survival of nerve cells, axon outgrowth and synaptogenesis). Monosialotetrahexosyl ganglioside is protective against neurodegeneration secondary to injury. The application of monosialotetrahexosyl ganglioside has a favorable effect on the improvement of cerebral hemodynamic parameters and the reduction of cerebral edema after injury. Monosialotetrahexosyl ganglioside reduces nerve cell edema by improving the activity of cell membrane enzymes. Animal experiments have shown that monosialotetrahexosyl ganglioside can imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/10C07H1/08
Inventor 胡增荣
Owner 胡增荣
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