Extraction method of monosialotetrahexosylganglioside
A technology of ganglioside and monosialic acid, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of burden on patients and their families, endangering patients' lives, and lack of specific drugs.
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[0023] 1. Extraction of total lipid
[0024] a. Weigh 5kg of pig brain, add 8 times the amount (40000ml) of chloroform-methanol mixture (1:3), use a high-speed tissue grinder to extract by electric stirring for 1 hour (speed 600 rpm), and let stand for 3 hours , and finally extract the supernatant, beat repeatedly three times, and combine 110L of the supernatant extracted three times.
[0025] b. Add 2500ml of water to every 10L of supernatant, stir for 1 hour, let it stand for 36 hours, and separate layers; separate the bottom chloroform layer to a total of 26L, recover after distillation and apply mechanically.
[0026] c. Separate 106 L of the supernatant aqueous phase, add 0.3% NaOH for hydrolysis, the pH is about 10, heat the water bath to boiling at 55°C±5°C for 40 minutes for hydrolysis.
[0027] d. After hydrolysis, it is detected by resorcinol, and it is purple, and the OD value is 0.2-0.35. It is cooled for later use.
[0028] 2. Coarse-porous resin exchange column...
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