One-step manufacture method of micro-flow diagnosis chip
A chip and microfluidic technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as complex procedures, achieve wide application prospects, short preparation time, and small non-specific adsorption effects
Inactive Publication Date: 2013-09-18
上海交通大学附属第一人民医院
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Problems solved by technology
However, the PDMS chip immobilized with biomolecules obtained in the above method has a planar structure, and the reaction pool is formed by bonding an additionally processed SU-8-based micropipe chip to it, which requires subsequent processing of the pipeline structure, and the process is complicated. If the production of microfluidic diagnostic chips and the immobilization of biomacromolecules can be completed in one step, the current process will be greatly simplified, the production process of microfluidic diagnostic chips will be accelerated, and the diagnostic chips will be brought to the market as soon as possible.
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Embodiment 1
[0026] Example 1: One-step preparation of human IgG microfluidic diagnostic chip
[0027] (1) Preparation of photoresist template
[0028] Firstly, SU-8-100 negative photoresist (Micro Chem, USA) was used to spin the glue on a single-sided polished silicon wafer at a speed of 2500r / min to obtain a photoresist with a thickness of 80 μm. The pre-baking condition is to rise from room temperature to 65°C for 10 minutes, hold for 10 minutes, then rise to 95°C for 30 minutes, hold for 35 minutes, and cool in the furnace. Exposure was carried out using a MA6 lithography machine from German Karl Suss Company, and the exposure time was 60s. The post-baking condition is to rise from room temperature to 90°C for 30 minutes, keep the temperature for 10 minutes, and cool in the furnace. Micro Chem's special SU-8 developer was used, and the development time was 6 minutes. After development, it was cleaned with isopropanol, and dried with nitrogen gas to obtain the required photoresist tem...
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The invention relates to a one-step manufacture method of a micro-flow diagnosis chip. The method comprises the step of fixing bio-macromolecule at a fixed point on the surface of a chip mould. The method is characterized in that the step of fixed-point fixation comprises the following specific steps: firstly, coating a anti-adhesion coating on the surface of the chip mould; spotting the sample of the bio-macromolecule solution at a special region; casting polydimethylsiloxane (PDMS); and demoulding a solidified PDMS casting layer, and uncovering the fixed bio-macromolecule. Therefore, By utilizing the method, the manufacturing chip and the fixation of the bio-macromolecule can be realized by one-step method, thus the required time of a technological process is rapidly and efficiently shortened, and the cost for manufacturing the micro-flow diagnosis chip is lowered.
Description
technical field [0001] The invention relates to the technical field of microfluidic diagnostic chips, in particular to a method for fixed-point immobilization of biomolecules on the inner surface of a chip to manufacture a microfluidic diagnostic chip. Background technique [0002] After more than ten years of development, microfluidic diagnostic chips have been widely used in the field of biochemical analysis, and micro-multivariate diagnostic chips have become a new development direction in future clinical diagnosis. The materials for making microfluidic diagnostic chips mainly include polymer materials such as PDMS (Polydimethylsiloxane, Polydimethylsiloxane), which have the advantages of convenient processing and molding, low price, and cheap mass production. [0003] In addition to making micro-pipe structures for reactions, microfluidic diagnostic chips often need to immobilize biomacromolecules inside the pipes for subsequent capture, separation, and biological reacti...
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IPC IPC(8): G01N33/543
Inventor 彭志海夏骏周崇治陈翔
Owner 上海交通大学附属第一人民医院