Analysis method for measuring pyrroloquinoline quinine content through ion pair chromatography

A technology of ion-pair chromatography and analysis method, which is applied in the direction of measurement device, analysis material, material separation, etc., can solve the problems of difficult popularization and application, high cost, and achieve the effect of effective separation and simple method

Inactive Publication Date: 2011-05-25
JIANGSU INST OF NUCLEAR MEDICINE
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

These analysis methods all require special instruments, and the cost is high, so it is not easy to popularize and apply.

Method used

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  • Analysis method for measuring pyrroloquinoline quinine content through ion pair chromatography
  • Analysis method for measuring pyrroloquinoline quinine content through ion pair chromatography
  • Analysis method for measuring pyrroloquinoline quinine content through ion pair chromatography

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Embodiment Construction

[0016] Chromatographic condition optimization

[0017] 1. Selection of solvent and detection wavelength

[0018] Dissolve in methanol, acetonitrile, chloroform, ethyl acetate, dimethyl sulfoxide (DMSO) and other solvents, and perform spectral scanning. In acetonitrile, chloroform, ethyl acetate and other solvents, PQQ has no obvious ultraviolet characteristic absorption peak; in methanol In the solvent, PQQ has three characteristic absorption peaks at 210nm, 248nm, and 365nm. In DMSO solvent, PQQ has two obvious characteristic absorption peaks at 256nm and 322nm, and the response value is 2.1 times that in methanol. DMSO was selected as the solvent, and 256nm with a higher response value was selected as the detection wavelength.

[0019] 2. Selection of mobile phase

[0020]

[0021] The buffer solution is: pH 2.8, 20 mM potassium dihydrogen phosphate solution containing 10 mM tetrabutylammonium bromide TABA.

[0022] The mobile phase does not add ion pair, and any propo...

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Abstract

The invention discloses an analysis method for measuring pyrroloquinoline quinine (PQQ) content through ion pair chromatography, and belongs to the technical field of chromatographic analysis. The PQQ produced through synthesis or fermentation is quantitatively analyzed by ion pair high performance liquid chromatography, the obtained regression equation is y=4E+06X+627846, R<2>=0.9995, and is linear in a test range of 2 to 12 mu g/mL, and the minimum detectable amount is 2.0ng (S/N is more than or equal to 3). The method is simple, and possibly generated impurities in the preparation process are effectively separated.

Description

technical field [0001] The invention discloses an analysis method for determining the content of pyrroloquinoline quinone by ion-pair chromatography, which belongs to the technical field of chromatography analysis. Background technique [0002] Pyrroloquinoline quinine PQQ is a new oxidoreductase prosthetic group with a molecular weight of 330. It is mainly distributed in prokaryotes, some plants and mammals, and its content is relatively high in milk, especially breast milk. The adjacent quinone and three carboxyl groups in its chemical structure are its important functional groups (the structural formula is shown below). As an electron acceptor or donor, it participates in the oxidation-reduction process catalyzed by enzymes, and can affect the function of the respiratory chain and the level of free radicals in the body. It is considered to be one of the essential vitamins in the body. It has various physiological functions such as promoting the growth of animal, plant an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34
Inventor 周杏琴张建康钦晓峰孔艳艳曹国宪
Owner JIANGSU INST OF NUCLEAR MEDICINE
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