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Synthesizing method of complete antigen of enramycin

A technology of enramycin and complete antigen, applied in the fields of organic chemistry and immunochemistry, to achieve the effect of simple synthesis steps

Inactive Publication Date: 2011-06-01
叶健强 +13
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no ideal reports on immunological detection methods for enramycin residues at home and abroad. Only the Japanese Ministry of Health and Welfare has reports on routine detection techniques for this residue in livestock and aquatic foods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of complete antigen of enramycin

[0032] Prepare liquid A: weigh 71.64g disodium hydrogen phosphate (Na 2 HPO4 2H2O), prepared into 1000mL solution with redistilled water, the concentration is 0.2mol / L, and stored at 4°C for later use;

[0033] Prepare liquid B: weigh 31.21g of sodium dihydrogen phosphate (NaH 2 PO4), make 1000mL solution with redistilled water, the concentration is 0.2mol / L, store it at 4℃ for later use;

[0034] Preparation of solution C: Take 72.0mL of solution A and 28.0mL of solution B in a 250mL Erlenmeyer flask to make a phosphate buffered saline solution (PBS) with pH 7.2, and store it at 4°C for later use;

[0035] Prepare D solution: use a pipette to absorb 0.25mL glutaraldehyde liquid, make it into a 100mL solution to obtain a 0.25% glutaraldehyde solution, and store it at 4°C;

[0036] Prepare liquid E: prepare sodium carbonate (Na 2 CO 3 ) 10g / L and disodium ethylenediaminetetraacetic acid (EDTA) 1mol / L alkaline EDTA s...

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PUM

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Abstract

The invention relates to a synthesizing method of a complete antigen of enramycin, belonging to the technical field of organic chemistry and immunochemistry. The synthesizing method comprises the following steps of: coupling the enramycin as a hapten with a carrier protein BSA (Bovine Serum Albumin) by using a glutaraldehyde method; and determining the coupling ratio of coupling substances by using a spectrophotometry. The invention successfully synthesizes an artificial antigen of the enramycin, adopts succinct and effective synthesizing step, is completely used for enzyme immnunoassay and provides a technological base for the fast detection, the easy and convenient operation and the cost reduction of related enzyme immnunoassay.

Description

technical field [0001] A method for synthesizing the complete antigen of enramycin, the invention belongs to the technical field of organic chemistry and immunochemistry, and the complete antigen of enramycin synthesized by the invention is used to prepare antibodies that specifically recognize the chemical veterinary drug enramycin and to develop highly efficient Immunoquick test kit for rapid detection of micro-content of enramycin. Background technique [0002] Since the invention of antibiotics, they not only have remarkable effects in the treatment and prevention of human diseases, but also play a huge role in animal husbandry feed additives. The growth-promoting effect of antibiotics on animals was discovered in the late 1940s by Stocktadt et al. Later, it was found that antibiotics such as tetracyclines, macrolides, and β-endocylamines were added to feed, all of which could promote growth and increase growth. weight, increase production, and improve feed remuneration...

Claims

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Application Information

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IPC IPC(8): C07K16/18G01N33/53
CPCY02P20/141
Inventor 叶健强严玉宝谢晶余华廖党金胡娟李江凌陈世界曹冶周岷江李红崔鹏博赵素君文豪
Owner 叶健强
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