Preparation method and application of radix astragali saponin

A technology of astragalus saponins and Astragalus membranaceus, which is applied in the field of preparation of astragalus saponins, can solve the problems of unclear active ingredients and limited effects, and achieve strong curative effect and be beneficial to clinical treatment

Active Publication Date: 2013-06-12
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Astragalus has the effect of dilating blood vessels, its effect is still very limited and the active ingredients are not very clear. The research work on the active ingredients of vasodilation in Astragalus has always been one of the important research topics in the field of traditional Chinese medicine.

Method used

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  • Preparation method and application of radix astragali saponin
  • Preparation method and application of radix astragali saponin
  • Preparation method and application of radix astragali saponin

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of astragaloside

[0033] Add 10 times the weight of Astragalus deionized water, heat and decoct for 3 hours, filter, and use the filtrate for later use; decoct the dregs and extract twice, the weight of water added is 8 times and 6 times the weight of Astragalus deionized pieces, and the extraction time is 2.5 times respectively. After 1 hour and 1.5 hours, filter, combine the filtrates, add ethanol to adjust the ethanol volume to account for 80% of the solution volume, carry out alcohol precipitation, and let stand for 12 hours. Recover ethanol from the supernatant under reduced pressure until there is no alcohol smell, dilute the resulting concentrated solution with 0.5 times the weight of water, filter, and slowly inject the filtrate into the treated HPD600 macroporous adsorption resin column, first elute with deionized water, Then use 2 times of column volume of 20% (volume / volume) aqueous ethanol to elute, discard the eluate of 20% (v...

Embodiment 2

[0044] Example 2: Preparation of rat isolated thoracic aortic ring

[0045] SPF grade male Sprague-Dawley (SD) rats, weighing 240-260 g, were used. Rapidly dissociate the rat thoracic aorta and place it in 95% O at 4°C 2 and 5% CO 2 In the K-H solution pre-saturated with mixed gas, the surrounding connective tissue was removed and cut into 4-5mm vascular rings, and the intima of the vascular rings was protected from damage during the process. According to the needs of the experiment, the inner surface of the vascular ring was rubbed with a cotton swab, and a part of the vascular ring was prepared as a model in which the endothelium was removed. Then the blood vessel ring is suspended in the bath tank of preset 10ml K-H solution, one end is fixed, and the other end is connected to the BIOPAC biological signal acquisition system (Biopac company, the U.S. by a tension transducer). 2 -5%CO 2 In the state of mixed gas, adjust its base tension to 2.0g, and stabilize it at 37°C f...

Embodiment 3

[0046] Example 3 Effects of Astragalus saponins on the relaxation and contraction function of isolated thoracic aortic rings in rats

[0047] 1. Observing the effect of total saponins of astragalus on the tension of isolated vascular rings in the basal state

[0048] Using the astragaloside prepared in Example 1, according to the cumulative dosing method, add astragaloside once every 8 to 10 minutes, and observe the effect of accumulatively administering 1 to 1000 mg / L of astragaloside on endothelial integrity and endothelial removal of vascular rings; control group Add K-H liquid instead of medicine in equal volume.

[0049] 2. Observing the effect of astragaloside on the tension of KCl and PE pre-contracted isolated vascular rings

[0050] In two states of intact and removed endothelium, observe the effect of cumulative concentration of total astragalosides (1-1000mg / L) on the tension of KCl (60mmol / L) and PE (1μmol / L) pre-contracted vascular rings; the control group was tr...

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Abstract

The invention provides a preparation method and application of radix astragali saponin. The preparation method of radix astragali saponin comprises the following steps: (1) adding water to radix astragali, extracting by decoction, filtering, and concentrating the filtrate; (2) adding ethanol to precipitate, and standing; (3) recovering the ethanol from the supernatant, diluting with water, injecting into macroporous adsorbent resin columns, and sequentially eluting with water, a 20% (volume / volume) ethanol water solution and a 70% (volume / volume) ethanol water solution; and (4) removing the ethanol in the eluent of the 70% (volume / volume) ethanol water solution, and drying to obtain the radix astragali saponin. The research indicates that the radix astragali saponin can activate the vascular endothelial cell NO-cGMP system, inhibit the calcium influx of vascular smooth muscle cells, and open the K[Ca] and K[ATP] channels, thereby relaxing the smooth muscles, especially vascular smoothmuscles; and therefore, the radix astragali saponin has an obvious function of vasodilatation, and is suitable for preventing and treating cardiovascular diseases, such as lowering the blood pressure, resisting angina pectoris and the like.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a preparation method and application of astragaloside, in particular to a preparation method and application of astragaloside which has the effect of relaxing blood vessels. Background technique [0002] Hypertension is one of the common and frequently-occurring diseases of the population in our country, and it is also an important factor in the formation of cardiovascular and cerebrovascular diseases. This is because the disorder of vasoconstriction and relaxation plays a leading role in the early occurrence, development and complications of cardiovascular diseases. effect. The incidence of hypertension is increasing day by day, which is extremely harmful to human health. At present, the drugs for the treatment of high blood pressure are mainly western medicines. Although they take effect quickly, they are highly dependent on drugs, require long-term medication, and may produce ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J53/00A61K31/7048A61P9/08A61P9/12A61P9/10
Inventor 屠鹏飞张可马旭马治中
Owner PEKING UNIV
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