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Kit for detecting gastric cancer susceptibility

A kit and susceptibility technology, which is applied in the field of kits for detecting susceptibility to gastric cancer, can solve problems such as changes and bad living habits, and achieve the effects of good repeatability, high sensitivity, and low detection cost

Inactive Publication Date: 2011-06-29
UNION STEMCELL & GENE ENG +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0009] The technical problem to be solved by the present invention is to comprehensively detect and analyze a group of genes and loci related to gastric cancer susceptibility, using specific primers and probes, and combining single nucleotide extension technology with microarray chip technology. Check whether the population carries the "gastric cancer susceptibility gene", screen out the gastric cancer susceptible population from the population, change bad living habits, and achieve the purpose of prevention

Method used

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  • Kit for detecting gastric cancer susceptibility
  • Kit for detecting gastric cancer susceptibility
  • Kit for detecting gastric cancer susceptibility

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Embodiment Construction

[0053] The present invention will be described in further detail below in conjunction with specific embodiments. For the scale-up of experiments without specific conditions indicated in the following examples, conventional conditions or the conditions suggested by the manufacturer are generally followed.

[0054] 1 DNA extraction:

[0055] Take the peripheral venous blood of the subject, add the same volume of cell lysate, and lyse twice to fully lyse the white blood cells. After centrifuging and discarding the supernatant, add proteinase K buffer and proteinase K (50ug / ml), incubate at 65°C for 10 minutes, iso Precipitate with propanol, wash twice with 75% ethanol, dry and dissolve in appropriate amount of TB solution.

[0056] 2PCR amplification

[0057] Take the subject's genomic DNA extract and add it to a 96-well plate for multiplex PCR amplification:

[0058] The total reaction volume is 5ul, of which 10M mol / L dNTPs 0.0375ul, 10×PCR Buffer0.5ul, 25mmol / L MgCl 2 1ul...

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Abstract

The invention discloses a kit for detecting the gastric cancer susceptibility, which is used for detecting four genes nearly concerned with gastric cancer as follows: MTHFR gene, CYP2E1 metabolic enzyme gene, XRCC1 and ADPRT repairase gene. The kit can simultaneously detect the following SNP (single nucleotide polymorphism) sites: the rs1801133 site of the MTHFR gene, the rs2031920 site of the CYP2E1, the rs25478 site of the XRCC1 gene and the rs1136410 site of the ADPRT gene. By using the kit disclosed by the invention, a group of genes and sites related to the gastric cancer susceptibility are detected, and whether the subject population carry gastric cancer susceptible genes can be judged clearly by using a specific primer and a specific probe by a mononucleotide extension technology combined with a microarray chip technology, thereby screening out the gastric cancer susceptible population from the subject population, changing unhealthy habits and customs and achieving the purpose of prevention.

Description

technical field [0001] The invention relates to a kit for detecting susceptibility to diseases, in particular to a kit for detecting susceptibility to gastric cancer, by detecting single nucleotide polymorphisms of MTHFR gene, CYP2E1 metabolic enzyme gene, XRCC1 and ADPRT repair enzyme gene locus (SNP) to predict the susceptibility of individuals to gastric cancer. Background technique [0002] Gastric cancer is one of the most common malignant tumors of the digestive tract, and is a common and frequently-occurring disease that seriously threatens human health and life. According to statistics, about 900,000 people around the world are newly diagnosed with gastric cancer every year. In my country, gastric cancer ranks first among digestive tract malignancies and third among systemic malignancies. According to the data released by the Health Information Statistics Center of the Ministry of Health in 2001, malignant tumors are the first cause of death among urban residents in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 韩俊领刘志霈周毓玲杜宏伟李楠靳霞刘蓉华
Owner UNION STEMCELL & GENE ENG
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