Method for rapidly propagating root and rhizome of Divaricate Gynura

Abstract

The invention discloses a method for rapidly propagating root and rhizome of Divaricate Gynura. The method comprises the following steps of: inducing culture, namely inoculating in a murashige and skoog (MS) start culture medium which does not contain any hormone so as to induce the generation of axillary buds; and proliferation and rooting culture, namely cutting a start seedling into single stem segments with buds, and inoculating in a culture medium, namely 1 / 2MS culture medium plus 0.2mg / L naphthylacetic acid (NAA) plus 20g / L sucrose plus 6g / L agar for synchronous proliferation and rooting, wherein culture conditions are both that the temperature is 25+ / -1 DEG C, the illumination intensity is 2000lx, and the illumination time is 16h / d. By the method, high-quality seedlings of the root and rhizome of Divaricate Gynura, which have pure seed character and are healthy and neat, can be rapidly, efficiently and massively produced.

Description

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a rapid propagation method of Panax notoginseng. Background technique

[0002] White-backed Sanqi (Gynura divaricata (L.) DC.) belongs to the genus Panax notoginseng of the Compositae family, also known as Baidongfeng, Baizicao, Yuloquat, Sanbaibang, Thick Noodles, Chicken Vegetables, Big Fat Beef, White Saffron, White Red cabbage, Fugui cabbage, Baizi cabbage, etc. are distributed from Taiwan to South China and Southwest China. Its roots, stems and leaves can be used as medicine; its roots are sweet and cool in nature, can clear away heat and cool blood, dissipate blood stasis and reduce swelling; effect. In Fujian and other places, folks use its stems and leaves to make tea and drink water to treat diabetes, etc., and the curative effect is very good. Panax notoginseng is rich in flavonoids and glycosides (Huang Qi et al., Determination of total flavonoid cont...

Examples

Embodiment 1

[0022] 1) Extraction and disinfection of explants: select young shoots about 20 days old as explants, cut off the leaves (keep the petioles), clean them with an appropriate amount of detergent, and then rinse them with running water for 1 hour. The clean workbench is sterilized with 70% alcohol for 15 seconds and 2% sodium hypochlorite for 12 minutes, rinsed with sterile water for 5 times, placed in an ultra-clean workbench, and cut into 0.5-1.0cm bud stem segments for later use.

[0023] 2) Induction culture: Inoculate the sterilized explants with buds on the stem segment in the ultra-clean workbench in the MS starting medium without any hormones to induce the production of axillary buds. The culture conditions are 25°C±1°C, and the light intensity is About 2000lx, photoperiod is 16h / 8h, obtains the high starting seedling of 5-8cm after about 20 days; ( figure 1 , A)

[0024] 3) Proliferation and rooting culture: use the terminal buds and budding stems of the starting seedli...

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a rapid propagation method of Panax notoginseng. Background technique

[0002] White-backed Sanqi (Gynura divaricata (L.) DC.) belongs to the genus Panax notoginseng of the Compositae family, also known as Baidongfeng, Baizicao, Yuloquat, Sanbaibang, Thick Noodles, Chicken Vegetables, Big Fat Beef, White Saffron, White Red cabbage, Fugui cabbage, Baizi cabbage, etc. are distributed from Taiwan to South China and Southwest China. Its roots, stems and leaves can be used as medicine; its roots are sweet and cool in nature, can clear away heat and cool blood, dissipate blood stasis and reduce swelling; effect. In Fujian and other places, folks use its stems and leaves to make tea and drink water to treat diabetes, etc., and the curative effect is very good. Panax notoginseng is rich in flavonoids and glycosides (Huang Qi et al., Determination of total flavonoid cont...

Examples

Embodiment 1

[0022] 1) Extraction and disinfection of explants: select young shoots about 20 days old as explants, cut off the leaves (keep the petioles), clean them with an appropriate amount of detergent, and then rinse them with running water for 1 hour. The clean workbench is sterilized with 70% alcohol for 15 seconds and 2% sodium hypochlorite for 12 minutes, rinsed with sterile water for 5 times, placed in an ultra-clean workbench, and cut into 0.5-1.0cm bud stem segments for later use.

[0023] 2) Induction culture: Inoculate the sterilized explants with buds on the stem segment in the ultra-clean workbench in the MS starting medium without any hormones to induce the production of axillary buds. The culture conditions are 25°C±1°C, and the light intensity is About 2000lx, photoperiod is 16h / 8h, obtains the high starting seedling of 5-8cm after about 20 days; ( figure 1 , A)

[0024] 3) Proliferation and rooting culture: use the terminal buds and budding stems of the starting seedli...

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