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Bacillus GZT and use thereof in degrading tribromophenol brominated flame retardant

A technology of bacillus and tribromophenol, which is applied in the field of microorganisms to achieve the effect of strong ability

Inactive Publication Date: 2012-08-22
GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the research of using Bacillus to degrade 2,4,6-TBP has not been reported

Method used

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  • Bacillus GZT and use thereof in degrading tribromophenol brominated flame retardant
  • Bacillus GZT and use thereof in degrading tribromophenol brominated flame retardant
  • Bacillus GZT and use thereof in degrading tribromophenol brominated flame retardant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The Bacillus GZT with the ability to degrade 2,4,6-tribromophenol according to the present invention is obtained by separating and purifying the sludge in the high-risk area of ​​electronic waste in Guiyu Town, Guangdong Province. The method of separation and purification is as follows: The domestication medium used is inorganic salt medium (g / L) (K 2 HPO 4 4.35, KH 2 PO 4 1.7, NH 4 NO 3 2.1, MgSO 4 0.2, MnSO 4 0.05, FeSO 4 ·7H 2 O 0.01, CaCl 2 ·2H 2 O 0.03, pH 7.0). First, add 3g of sludge to 100ml of inorganic salt medium containing 10mg / L of 2,4,6-tribromophenol. After acclimation at 37°C for 60 days, transfer it to contain 2,4,6-tribromophenol at a volume percentage of 10%. The 15mg / L inorganic salt medium was then acclimated for one week, and so on, the concentration of 2,4,6-tribromophenol was gradually increased for acclimation. The concentration of 2,4,6-tribromophenol was 20mg / L and 30mg respectively. / L, 50mg / L. After the completion of the acclimatization...

Embodiment 2

[0034] A single colony of Bacillus GZT was inoculated into 3ml beef extract peptone medium and cultured for 15h (OD 600 =0.8) Inoculate 10% by volume in an inorganic salt culture medium containing 2mg / L 2,4,6-tribromophenol, where the pH of the inorganic salt is 7.5, and react at 35°C and 200rpm in a shaker 120h, regular sampling was used to determine the degradation rate and debromination rate by high performance liquid chromatography and the mineralization rate of 2,4,6-tribromophenol by gas chromatography. The determination of the degradation rate is the same as in Example 1; the determination of the debromination rate is to determine the bromide ion concentration, which is determined by the bromophenol red method with a spectrophotometer, the debromination rate = the bromide ion concentration generated / the bromine concentration of the substrate; -The detection of tribromophenol mineralization rate is as follows: through the mineralization product CO 2 Sampling to detect the ...

Embodiment 3

[0036] A single colony of Bacillus GZT was inoculated into 3ml beef extract peptone medium and cultured for 15 hours. The inoculum was 30% by volume into an inorganic salt medium containing 3mg / L 2,4,6-tribromophenol. The pH value of the inorganic salt was 6.5, and the reaction was carried out on a shaker at 20°C and 200 rpm for 96 hours. Samples were taken regularly to determine the degradation rate, debromination rate, and mineralization rate. The determination method is the same as above. The measured degradation rate was 93.4%, the debromination rate was 87.9%, and the mineralization rate of 2,4,6-tribromophenol was 22.2%.

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Abstract

The invention discloses Bacillus GZT and use thereof in the field of environment protection, particularly the field of degradation of 2,4,6-tribromophenol. The Bacillus GZT was separated and purified from sludge in electronic waste high risk areas and was collected in China Center for Type Culture Collection on December 13th, 2010, with a collection number of CCTCC M2010346. The Bacillus GZT has high 2,4,6-tribromophenol degrading capacity, the degradation rate of the Bacillus GZT reaches 96.2 percent, the bromine removing rate reaches 88.4 percent, and the mineralization rate reaches 29.0percent. The Bacillus GZT can be used in the field of environment protection and can be used for degrading 2,4,6-tribromophenol.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and particularly relates to a Bacillus GZT and its application in the field of environmental protection, especially the biodegradable brominated flame retardant (BFR) 2,4,6-tribromophenol. Background technique [0002] With the rapid development of the electronic information industry in recent years, brominated flame retardants (BFR) are widely used in daily life and industrial production, and their lipophilicity, environmental stability, non-degradability and biomagnification have made them become Concerned about a type of organic pollutants with potential health hazards. 2,4,6-Tribromophenol (2,4,6-TBP) as an intermediate product of BFR is widely used in electronic manufacturing and other fields. In 2001, the global output reached 9,500 tons / year. According to reports, TBP is a brominated phenolic compound with acute toxicity, which was classified as hazardous waste by the Environmental Prot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/07A62D101/28
Inventor 安太成祖蕾李桂英
Owner GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI