Bacillus pumilus strain.Bap9 capable of effectively degrading benzo(a)pyrene and application thereof
A technology of Bacillus pumilus and benzo, which is applied in the field of environmental pollutant treatment, can solve the problems of low biodegradation efficiency of benzo[a]pyrene, and achieve the effect of improving the degradation rate
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Embodiment 1
[0052] Example 1: Isolation of strain Bacillus pumilus strain.Bap9 and performance of degrading benzo[a]pyrene
[0053] 1. Separation and purification method for efficiently degrading benzo[a]pyrene strain Bap9
[0054] The method has the following steps:
[0055] 1. Weigh 10g of soil sample and add it into a conical flask equipped with 100mL acclimation medium. The effective ingredients of the acclimatization medium are: (NH 4 ) 2 SO 4 1g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.1g, FeCl 3 0.5g, CaCl 2 0.5g, benzo[a]pyrene 0.04g, distilled water 1000mL, pH 7.0;
[0056] 2. Place the above-mentioned Erlenmeyer flask in a shaker at 160r / min and 37°C for 1 week, transfer it to fresh acclimatization medium according to 10% inoculum amount, and continue to culture for 1 week, repeating 3 times;
[0057] Draw 1mL of the culture solution after repeated acclimation, pick colonies with different characteristics, vigorous growth and stability from the plate, and repeate...
Embodiment 2
[0061] Example 2: Effects of different initial pH values on the growth and degradation of benzo[a]pyrene of bacterial strain Bacillus pumilus strain.Bap9
[0062] Regulate inorganic salt medium (the composition of this medium is: (NH 4 ) 2 SO 4 1g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.1g, FeCl 3 0.5g, CaCl 2 0.5g, benzo[a]pyrene 0.04g, distilled water 1000mL) pH values were 5.5, 6.0, 7.0, 8.0, 9.0, 9.5 respectively, and the growth situation of bacterial strain Bap9 and the degradation performance to benzo[a]pyrene were observed. The initial concentration of benzo[a]pyrene was 40 mg / L, the inoculum size was 5% (V / V), 160 r / min, 37 ℃ shaker cultured for 7 days, and the bacterial OD value and benzo[a]pyrene were measured. residual rate, the result is as figure 2 .
[0063] Depend on figure 2 It can be seen that when the pH is 8.0, the degrading bacteria have the strongest degradation effect on benzo[a]pyrene, and the degradation rate is 10.92% after 7 da...
Embodiment 3
[0064] Example 3: Effects of different inoculum sizes on the growth and degradation of benzo[a]pyrene of the bacterial strain Bacillus pumilus strain.Bap9
[0065] In the inorganic salt medium (the composition of this medium is: (NH 4 ) 2 SO 4 1g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.1g, FeCl 3 0.5g, CaCl 2 0.5g, benzo[a]pyrene 0.04g, distilled water 1000mL, pH value 7.0), the residual rate of benzo[a]pyrene after 7 days was 98.31%, 94.26%, 91.75%, 89.94% and 92.86% respectively ( See image 3 ). Depend on image 3 It can be seen that within a certain range, the larger the inoculation amount, the higher the bacterial density, and the higher the degradation rate of the bacterial strain within a certain range, but the larger the inoculation amount, the better. When the inoculum amount is 10.0%, the specific inoculum amount is 5.0%. low degradation rate.
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