Real time detecting apparatus and method for microbial activity in air

Abstract

The invention provides a real time detecting apparatus and a method for microbial activity in air. The real time detecting apparatus comprises a sampling pump, a fixing base, a fixing cannula, a detecting tube and a luminescence apparatus. The method comprises that: a desiring place is divided into a plurality of detection areas; with the sampling pump, indoor air is introduced into the fixing cannula through an air inlet pipe provided on the fixing cannula in each detection area, such that microbes in air adhere to a sterilized cotton stick of the detecting tube provided in the fixing cannula; after sampling, a transparent cannula of the detecting tube is sleeved on the sterilized cotton stick; a sealed column in one side of the detecting tube is broken, such that the sterilized cotton stick is immersed in a luciferase agent; the detecting tube is placed into a detecting aperture of the luminescence apparatus to analyze relative luminous quantity of the detecting tube through the luminescence apparatus; detected values of each area in the detected place are input into a computer; the detected values are collected through the computer to complete an isogram of the detected resultsto quickly obtain various amounts of the microbes in air in each area in the detected place area.

Description

technical field [0001] The present invention relates to a device and method for real-time detection of microbial activity in the air, in particular to a method for dividing a place to be detected into multiple areas, using detection tubes to collect samples of microorganisms in the air, and then placing each sample under luminescent light In the instrument, the luminescence of the sample is analyzed in real time by the cold light meter, and then input into the computer to obtain the isopleth map, and the method for quickly knowing the amount of microorganisms in the air in each area of ​​the detection site. Background technique [0002] There are many microorganisms (such as bacteria) in the air, in order to detect the amount of bacteria contained in the air of indoor places. The current air detection method mainly uses impact sampler 1 (such as figure 1 shown) for sample collection. Before sampling, the detector must conduct indoor site investigation and on-site observati...

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Problems solved by technology

[0003] However, in the existing detection method, the detection personnel must have professional training, and the detection time needs to be as long as more than 48 hours, which is extremely time-consuming, troublesome, and the detection cost is extremely expensive; Expensive, so most of them only detect one point in a space as a reference, and the growth of different microbial species is different, the inspector must have the ability to distinguish bacteria and fungi, so counting is more difficult

Existing detection methods use quantitative detection methods, and the detection results cannot obtain the best accuracy

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