Separation and enrichment method of total flavones and total alkaloids of Lotus Plumule

A technology of total alkaloids and enrichment of lotus seeds, which is applied to medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, can solve the problems of low comprehensive utilization of medicinal materials, high toxicity, and cumbersome purification steps. The materials are cheap and easy to obtain, the comprehensive utilization rate is high, and the yield rate is high

Inactive Publication Date: 2011-11-02
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of the existing separation and purification methods for lotus root alkaloids and flavonoids, which have high toxicity, cumbersome purification steps, and low comprehensi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 100 g of the lotus seed heart medicinal material, reflux extract with 70% (mass percentage concentration) ethanol 8 times the mass of the medicinal material, and filter for 60 min. Medicinal dregs is reflux extracted with 70% (mass percentage concentration) ethanol that is 10 times the mass of the medicinal material for 45 min, filtered, combined filtrate, concentrated under reduced pressure to 5 times the mass of crude drug, treated with chitosan for impurity removal, and filtered to obtain Miscellaneous fluid.

[0023] Put 30mL of the pretreated D152 resin into a glass column with an inner diameter of 15mm and a height of 40mm, add the lotus seed heart impurity removal solution prepared above, purify water with about 5 times the column volume, and wash away the water-soluble Impurities. Then it was eluted with 10BV of 70% ethanol at a flow rate of 2BV / h, and the eluate I was collected. Then use 5BV of 70% (mass percent concentration) ethanol solution with a NaC...

Embodiment 2

[0025] Take 1000g of the lotus seed heart medicinal material, reflux extract twice with 60% (mass percentage concentration) ethanol 10 times the mass of the medicinal material, combine the extracts, recycle the ethanol until complete, add purified water to make it 3 times the mass of the crude drug, and use ZTC1 +1-Type II clarifier for impurity removal treatment and filtration to obtain impurity removal liquid.

[0026] Put 300mL of the pretreated D152 resin into a glass column with an inner diameter of 15mm and a height of 40mm, and add the lotus seed heart impurity removal solution prepared above. Purify water with about 5 times column volume, and wash away water-soluble impurities at a flow rate of 2BV / h. 10BV of 70% ethanol was eluted at a flow rate of 2BV / h, and the eluate I was collected. Then use 7BV of 70% ethanol solution with NaCl content of 0.01g / mL to elute at a flow rate of 2BV / h, and collect the eluate II. After the eluent I was dried, the total flavonoids cru...

Embodiment 3

[0028] Take 1000 g of the lotus seed heart medicinal material, reflux extract twice with 70% (mass percentage concentration) ethanol 12 times the mass of the medicinal material, combine the extracts, recover the ethanol until complete, add purified water to make up to 5 times the mass of the crude drug. Use ZTC1+1-II clarifier to remove impurities, filter to obtain impurity removal liquid.

[0029] Put 300mL of pretreated D001-CC cationic resin into a glass column with an inner diameter of 15mm and a height of 40mm. Add the lotus seed heart impurity removal liquid prepared above. Purify water with about 5 times column volume, and wash away water-soluble impurities at a flow rate of 2BV / h. 5BV of 50% ethanol was eluted at a flow rate of 2BV / h, and the eluate I was collected. Then use 10BV of 50% (mass percent concentration) ethanol solution with a NaCl content of 0.05g / mL to elute at a flow rate of 2BV / h, and collect the eluate II. After the eluent I was dried, the total fla...

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Abstract

The invention relates to the technique for separating and purifying effective parts from Lotus Plumule, and specifically discloses a separation and enrichment method of total flavones and total alkaloids of Lotus Plumule. According to the separation and enrichment method, alkaloids and flavones of Lotus Plumule are separated in a same separation unit in a centralized way and then separately eluted and enriched with different eluents. The separation and enrichment method comprises the following specific steps of: carrying out ethanol/water extraction on Lotus Plumule used as the raw material and removing impurities with a clarifying agent; enriching and separating the purified liquid with macroporous weak-acid cation exchange resin, eluting with ethanol/water to obtain total flavones, concentrating the eluate to be dry and drying to obtain total flavones of Lotus Plumule; and then eluting with NaCl-containing ethanol/water to obtain the total alkaloids, concentrating the eluate to be dry, removing salts and drying to obtain the total alkaloids. The separation and enrichment method provided by the invention is simple, feasible and environmentally-friendly, and has the characteristics of low cost, high yield of total flavones and total alkaloids; and by the adoption of the method, Lotus Plumule resources can be saved greatly, and the pharmaceutical utilization rate of Lotus Plumule can be improved.

Description

technical field [0001] The invention belongs to the technology of separating and purifying effective parts from lotus seeds, and in particular relates to a method for separating and enriching total flavonoids and total alkaloids of lotus seeds. Background technique [0002] The lotus seed heart is the green germ and radicle of the mature seeds of the water lily plant lotus (Nelumbo nucifera Gaertn.). According to "Compendium of Materia Medica", it can "clear the heart and remove heat", while "Suixiju Diet Spectrum" records that it tastes "bitter and cool", and can "contract liquid, stop sweating, reduce heat, refresh the mind, stop bleeding, and solidify essence" , Modern pharmacological analysis proves that it has the effect of strengthening the heart and lowering blood pressure, and reversing multi-drug resistance of tumors. [0003] Studies have found that lotus seeds contain a variety of chemical components, mainly alkaloids, flavonoids, polysaccharides and volatile oil...

Claims

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Application Information

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IPC IPC(8): A61K36/62A61P9/12A61P9/06A61P7/02A61P3/10A61P35/00A61P39/06A61K131/00
Inventor 刘韶雷鹏李新中尹桃
Owner CENT SOUTH UNIV
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