Application of taurine in preparing medicament for preventing nerve cell damage caused by dependence producing medicaments
A technology for nerve cell damage and addictive drugs, applied in drug combinations, nervous system diseases, active ingredients of anhydride/acid/halide, etc., to reduce apoptosis and enhance oxidation activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] The establishment of embodiment 1METH-induced behavioral sensitization animal model and the determination of taurine content
[0038] 1. Experimental animals: The animals used for modeling in this experiment are male Wistar rats with a weight of 180-200g. All the animal operations in this experiment conform to the requirements of AAALAC. After 5 days of feeding and adaptation, the animals are randomly divided into experimental groups And the control group, 10 animals in each group, the animals in the experimental group were subcutaneously injected with 2.5 mg / kg METH at a dose volume of 0.1%, and the animals in the control group were injected with normal saline subcutaneously. According to literature reports, administration at intervals of 10 hours can better simulate the dosing habits of methamphetamine dependents, so the administration is administered twice a day, each at 8:00 am and 6:00 pm every day, for 7 consecutive days.
[0039] 2. Detection
[0040] (1) Detect...
Embodiment 2CC
[0050] Example 2 The CCK-8 method detects the influence of different concentrations of taurine on PC12 cells damaged by METH, amphetamine and cocaine and C6 cells damaged by morphine
[0051] 1. Experimental materials
[0052] PC 12 cells are a clonal cell line derived from a rat adrenal medullary pheochromocytoma. Under normal conditions, PC 12 cells are similar to adrenal medullary cells not only in morphology, but also in many aspects of physiology and biochemistry. After adding nerve growth factor (NGF), PC 12 cells differentiate into sympathetic neurons in morphology , accompanied by physiological and biochemical changes, eventually leading to the neuron-like function of PC 12 cells. Because of its ability to be subcultured, as a neuron cell model, it is widely used in the research of neurophysiology, pathology and pharmacology. The C6 cell line, derived from glioma, exhibits the properties of astrocytes, expresses mu-opioid receptors on the membrane surface, and the in...
Embodiment 3
[0079] Example 3 Effects of Taurine on CAT and GPx Activities of PC12 Cells Damaged by METH and C6 Cells Damaged by Morphine
[0080] According to the instructions of the catalase kit and the glutathione kit, the activities of catalase (CAT) and glutathione peroxidase (GPx) were detected on the intracellular proteins of PC12 cells and C6 cells, respectively. .
[0081] Test results such as Figure 13 and Figure 14 shown. Depend on Figure 13 It can be seen that for METH-induced PC12 cells, compared with the non-taurine protection group, the activity of CAT in the taurine-protected PC12 cell group was significantly increased (P Figure 14 It can be seen that for C6 cells induced by morphine, compared with the group without taurine protection, the CAT activity and GPx activity of the taurine-protected C6 cell group were significantly increased (P<0.05).
[0082] Experiments have proved that taurine can reduce nerve cell damage caused by METH and morphine through oxidative da...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More