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High-efficiency organic pollutant carbendazim degrading bacteria and use thereof

A technology of carbendazim and microbial strains, applied in the restoration of contaminated soil, bacteria, and methods based on microorganisms, which can solve problems such as carcinogenicity, mutagenesis, and chromosomal aberrations

Inactive Publication Date: 2012-11-07
孙建光
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the residual period of carbendazim in the soil is relatively long, which can cause human liver disease, lead to chromosomal aberration, and has potential carcinogenic, teratogenic, and mutagenic effects, and is toxic to mammals. Therefore, the degradation of carbendazim is studied becomes more important
[0005] At present, there are relatively few research reports on carbendazim-degrading bacteria at home and abroad

Method used

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  • High-efficiency organic pollutant carbendazim degrading bacteria and use thereof
  • High-efficiency organic pollutant carbendazim degrading bacteria and use thereof
  • High-efficiency organic pollutant carbendazim degrading bacteria and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1, Isolation and identification of carbendazim-degrading bacteria DJL22

[0034] 1. Isolation of carbendazim-degrading bacteria DJL22

[0035] Add 10 g of soil samples (collected from farmland soil polluted by carbendazim in Beijing, China) to 100 ml of enriched culture solution with a carbendazim concentration of 100 mg / L, culture with shaking at 25°C and 200 r / min for 3 days, and transfer 5 ml of the culture solution to the Connected to 200 mg / L enrichment culture solution with carbendazim concentration, cultivated for 3 days, enriched successively, and transferred 5 times, so that the concentration of carbendazim was 100, 200, 400, 500, 800 and 1000 mg / L. After the enrichment is completed, take the culture solution and spread it evenly on the same concentration of carbendazim separation medium and inorganic salt medium, and cultivate at a constant temperature of 25°C. After a single colony appears on the plate, pick a larger single colony and transfer it to ...

Embodiment 2

[0056] Example 2, Quantitative determination of the ability of Mycobacterium sp. DJL22 CGMCC No.5041 to degrade carbendazim

[0057] 1. Preparation of standard curve for determination of carbendazim

[0058] Prepare a series of standard suspensions with carbendazim standard substance (purchased from Sigma company), according to the feature that carbendazim has a maximum absorption peak at 281nm, measure OD281 by ultraviolet spectrophotometry, and then draw carbendazim assay standard curve, The OD281 determination results of different concentrations of carbendazim are shown in Table 2:

[0059] Table 2 OD281 values ​​of different concentrations of carbendazim

[0060]

[0061] According to the data in Table 1, the equation of the standard curve for the determination of carbendazim is y=0.0769x+0.0064, R 2 = 0.999. Carbendazim assay standard curve as figure 1 shown.

[0062] 2. Quantitative determination of the ability of Mycobacterium sp. DJL22 CGMCC No.5041 to degrade ...

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Abstract

The invention discloses high-efficiency organic pollutant carbendazim degrading bacteria and use thereof. The organic pollutant carbendazim degrading bacteria are (mycobacterium sp.)DJL22, and were collection in China General Microbiological Culture Collection Center, and the collection number of organic pollutant carbendazim degrading bacteria is CGMCC No.5041. When the (mycobacterium sp.)DJL22 CGMCC No.5041 is cultured in inorganic salt culture medium for 5 days, the rate of degradation of carbendazim at a concentration of 100mg / L reaches 84.79 percent; and when the (mycobacterium sp.)DJL22CGMCC No.5041 is cultured in inorganic salt culture medium for 15 days, the rate of degradation of carbendazim at a concentration of 600mg / L reaches 62.77 percent. Therefore, the stain can efficiently degrade carbendazim and has a bright application prospect in repairing soil polluted by carbendazim.

Description

technical field [0001] The invention relates to a bacterium for efficiently degrading organic pollutant carbendazim and its application. Background technique [0002] The chemical name of carbendazim is N-(2-benzoimidazolyl)-methyl carbamate, and the English chemical name is methyl benzimidazol-2-ylcarbamate. Molecular formula is C 9 h 9 N 3 o 2 , the structural formula is as follows. [0003] [0004] Carbendazim can interfere with the process of nucleic acid synthesis and cell mitosis, and has the effect of preventing and controlling fungal diseases of various crops. It is currently the main fungicide for preventing and controlling plant diseases in agriculture. It has a wide range of applications and mainly acts on rice, peanuts, cotton, and tobacco. , beets, and a variety of vegetables, can prevent melon powdery mildew, tomato early blight, bean anthracnose, blight, rape sclerotinia, green onions, leek gray mold, eggplant, cucumber sclerotinia, melons, bean anthr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/32A62D101/04A62D101/28
Inventor 孙建光
Owner 孙建光
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