Method for extracting pannonibacter phragmitetus from sludge

A technology of microorganisms and sludge, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of weak competitiveness and high cost of exogenous microorganisms, and achieve the effects of long duration of high temperature, low cost, and simple cultivation operation

Active Publication Date: 2014-05-28
SUN YAT SEN UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method that fully utilizes the indigenous microorganisms in the sludge to promote composting, and the conditions in the composting process are stable and controllable according to the deficiencies in the existing mature sludge composting treatment, which overcomes the existing sludge composting process. The introduction of exogenous microorganisms in composting technology has the disadvantages of weak competitiveness and high cost, and realizes the environmental, economic and social benefits of recycling organic waste

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  • Method for extracting pannonibacter phragmitetus from sludge

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Embodiment 1

[0022] Mix the dewatered sludge and decomposed sludge according to the weight ratio dehydrated sludge: decomposed sludge=4:1, add 0.8% normal saline, and the weight-volume ratio (g / ml) of sludge to normal saline is sludge: Physiological saline = 1:10, shaken for 1 hour, obtained after filtration; ordinary nutrient solution in enriched culture medium: sludge exudate = 4:1. Indigenous microorganisms obtained from enrichment culture, the effective number of viable bacteria (CFU) is ≥10 9 / g, and it is added to the heap as a compost starter, wherein the initial sludge moisture content is 55%, and the weight ratio is: sludge: wood chaff = 1:0.1, and the static forced ventilation method is Intermittent ventilation, the frequency is 15 minutes per hour, and the ventilation volume is 0.04~0.09m 3 / min·t, the duration is 15d. The test found that the initial organic matter of the compost was 61.4%, the organic matter of the compost was 37.6%, the organic matter degradation rate was 38...

Embodiment 2

[0024] Mix the dewatered sludge and decomposed sludge according to the weight ratio of dehydrated sludge: decomposed sludge = 4:1, add 0.8% physiological saline, and the weight-volume ratio (g / ml) of sludge to physiological saline is: sludge : Physiological saline = 1:10, shake for 1 hour, get the filtrate after filtration; enrich the ordinary nutrient solution in the culture medium: sludge exudate = 4:1. Indigenous microorganisms obtained from enrichment culture, the effective number of viable bacteria (CFU) is ≥10 9 / g, and it is added to the heap as a compost starter, wherein the initial sludge moisture content is 55%, and the ratio by weight is: sludge: conditioning agent = 1:0 mixed, the static forced ventilation method is Intermittent ventilation, the frequency is 15 minutes per hour, and the ventilation volume is 0.03~0.08m 3 / min·t, the duration is 15d. The test found that the initial organic matter of the compost was 33.7%, the organic matter of the compost was 26.4...

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Abstract

The invention discloses a method for extracting pannonibacter phragmitetus from sludge, which comprises: mixing dewatered sludge with aged sludge, adding physiological saline, shaking, filtering, collecting filtrate and obtained mixed extract of pannonibacter phragmitetus; performing enrichment culture in enrichment culture solution to enrich pannonibacter phragmitetus; adding the pannonibacter phragmitetus into compost sludge; and composting by regulating compost process parameters and static forced ventilation fermentation. Compared with external microorganisms, the pannonibacter phragmitetus extracted by the method has the advantages that: the resource is wide; the culture operation is easy; the competitive power is huge; the adaptability to compost environment is high; the fermentation of compost can be started even when the environment temperature is low; the compost efficiency is improved; and the cost is reduced. When the pannonibacter phragmitetus is used in composting, the process parameters are stable and the practicality and stability of the invention can be further improved.

Description

technical field [0001] The invention relates to the field of solid waste treatment, in particular to a method for extracting indigenous microorganisms from sludge. Background technique [0002] With the improvement of the degree of urbanization, the sewage treatment plants are further rebuilt and expanded, so that the amount and degree of sewage treatment are also rapidly increased, and the amount of sludge produced is increasing day by day. Taking Guangzhou City as an example, there are currently 12 urban sewage treatment plants, the actual sewage treatment capacity is 1.59 million tons per day, and the production of wet sludge (with a moisture content of about 80%) is 900 tons. It is estimated that by 2020, Guangzhou The amount of dry sludge in the city reaches 760 tons per day. Urban sewage sludge contains a large amount of organic matter, nitrogen, phosphorus, potassium and various trace elements required for plant growth and development. By composting the sludge, it is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C05F17/00
CPCY02W30/40
Inventor 张淑娟江纪修林亲铁黄柳祯章婷婷张旭兰
Owner SUN YAT SEN UNIV
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