A kind of preparation method of bacillus thuringiensis liquid fermentation medium

Abstract

The invention relates to a preparation method of a liquid fermentation medium for bacillus thuringiensis, which includes mushroom residue preprocessing and culture medium ingredient supplementation. The preparation method disclosed by the invention includes the following steps: strong acid is utilized to degrade the cellulose and pleurotus eryngii mycelium in mushroom residue into sugar and a small amount of protein, phosphorus and trace elements as main nutrient substances contained in residue extract, which can be utilized by the bacillus thuringiensis, meanwhile, nitrogen source and inorganic salt ions are supplemented partially, the bacillus thuringiensis can grow and reproduce under the condition of maintaining appropriate pH, temperature and ventilation, so that insecticidal active substances, such as spores and insecticidal crystal protein, can be produced, and the fermentation medium is concentrated and dried to form an environment-friendly and secondary pollution-free microbial insecticide product. The preparation method not only solves the problem of mushroom residue processing and prevents the mushroom residue from polluting the environment, but also realizes the comprehensive utilization of resources, and meanwhile, the preparation method also greatly reduces the production cost of the microbial insecticide, and turns waste into treasure.

Description

technical field [0001] The invention relates to a method for preparing a microbial culture medium, in particular to a method for preparing a liquid fermentation medium for Bacillus thuringiensis, and belongs to the technical field of biological pesticides. Background technique [0002] Bacillus thuringiensis ( Bacillus thuringiensis , referred to as Bt) is a Gram-positive bacterium that can produce insecticidal crystals with specific insecticidal activity against various pests such as Lepidoptera, Diptera, Coleoptera, Homoptera, and nematodes while forming spores Proteins (Insecticidal Crystal Proteins, ICPs). These insecticidal crystal proteins have the advantages of being harmless to humans and animals, and being environmentally friendly. Therefore, Bacillus thuringiensis is widely used in pest control in fields such as agriculture, forestry and public health, and has become the most widely used and most productive microbial insecticide in the world. At present, the mai...

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Problems solved by technology

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a method for preparing a Bacillus thuringiensis liquid fermentation medium, which can prepare bacterial chaff into a biological pesticide with higher added value through simple treatment and fermentation process, which can solve the problem of bacterial chaff environmental pollution problems, realized its resource utilization and final disposal, and also reduced the production cost of biopesticides, turning waste into treasure

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