Cultivation method of handle tree tongue

A cultivation method, the technology of tree tongue, applied in the field of artificial cultivation of medicinal fungi, can solve the problems of high price and depletion of wild resources, and achieve the effect of small investment, fast growth and easy promotion

Inactive Publication Date: 2011-12-28
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the destruction of the ecological environment, coupled with the high price of the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Ganoderma gibbosum (Nees) Pat strain was purchased from Guangdong Institute of Microbiology, strain number GIM5.6.

[0057] Inoculate the mycelium of T. pedunica on the mother seed medium and expand the culture for 12 days to obtain the mother species of T. pedunica, inoculate the mother species on the original seed medium and cultivate it for 18 days to obtain the original seed, and connect the original seed to the section wood for segmenting. Wood cultivation mushroom cultivation;

[0058] Ordinary PDA medium for mother seed medium: 200 grams of potato, 20 grams of glucose, 15-20 grams of agar, 1000 ml of water; original seed medium: 80% of broad-leaved wood chips, 18% of wheat bran, 1% of sucrose, 1% of gypsum powder %, add water after stirring evenly, and the water content of the final medium reaches 55-60%; the original seed medium is made into a 500mL original seed bottle;

[0059] The culture conditions of the mother species and the original species are all cult...

Embodiment 2

[0072] The species Ganoderma gibbosum (Nees) Pat was isolated from the fruiting body of the wild tree tongue, and the strain was isolated by tissue separation method, that is, the fruiting body of the wild tree tongue was cut into two parts with a sterile scalpel. Half, use a knife to cut out a small square tissue of about 0.3cm, use tweezers to place the bacterial flesh tissue in the center of the PDA medium of the petri dish, after 2-3 days of culture, white hyphae can be seen growing from the tissue block, transfer the tube 1-2 times to obtain Purified mycelia of G. pedunata.

[0073] Inoculate the mycelia of T. pedunica on the mother seed medium and expand the culture for 13 days to obtain the mother species of T. pedunica, inoculate the mother species on the original seed medium and cultivate it for 15 days to obtain the original seed, and inoculate the original seed on the substitute material for substituting Cultivation and fruiting culture of material; the common PDA m...

Embodiment 3

[0095] The species Ganoderma gibbosum (Nees) Pat was isolated from the fruiting body of the wild tree tongue, and the strain was isolated by tissue separation method, that is, the fruiting body of the wild tree tongue was cut into two parts with a sterile scalpel. Half, use a knife to cut out a small square tissue of about 0.5cm, and use tweezers to place the bacterial flesh tissue in the center of the PDA medium in the petri dish. After 3 days of cultivation, white hyphae can be seen growing from the tissue block. Transfer the tube 1 or 2 times to obtain purified Mycelium of stalked tree tongue.

[0096] Inoculate the mycelium of T. pedunica on the mother seed medium and expand the culture for 10 days to obtain the mother species of T. pedunica, inoculate the mother species on the original seed medium and cultivate it for 25 days to obtain the original seed, and inoculate the original seed on the section wood for sectioning. Wood cultivation mushroom cultivation;

[0097] Us...

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PUM

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Abstract

The invention discloses a culture method for ganoderma applanatum, which comprises the following steps of: inoculating the ganoderma applanatum hyphae into a mother seed culture medium for amplification culture, obtaining the ganoderma applanatum mother seed, inoculating the mother seed into a raw seed culture medium to obtain a raw seed and inoculating the raw seeds into basswood to carry out basswood culture or onto replacing materials to carry out replacing material culture fruiting culture. Through being metered by 1000kg wood, 42 to 67kg of fruit bodies can be ordinarily harvested in the current year, and 25 to 33kg of fruit bodies can be harvested in the second year; and through being metered by 1000kg replacing materials, 50 to 72 kg of fruit bodies can be ordinarily harvested in the current year. The culture method is simple and convenient and is easy to implement, the investment is low, the effect is fast taken, the popularization is easy, and the defect of insufficient wild resources is overcome. The method of the invention has the advantages that the hypha growth speed is high, the daily average growth speed of the hyphae can reach 16mm/d, the biological efficiency of the fruit bodies can reach 6.7 percent to 10 percent, the fruit body yield is high, and the quality is good.

Description

technical field [0001] The invention relates to a method for artificial cultivation of medicinal fungi, in particular to a method for artificial cultivation of tongue stalk. Background technique [0002] Tree tongue with handle, scientific name Ganoderma gibbosum (Nees) Pat, also known as Ganoderma gibbosum (Nees) Pat, also known as Ganoderma lucidum and old mother fungus, is a Ganoderma fungus of the Ganodermaceae Ganodermataceae, distributed in Jiangsu, Zhejiang, Hainan, Fujian, Guangxi and other places, mainly in Hainan, It is used to treat stomach problems in Hainan folk, and its other effects are similar to those of G.applanatum. Foreign countries are mainly distributed in Indonesia. In recent years, due to the destruction of the ecological environment and the high price of the wild tree tongue on the market in Hainan, which has led to over-harvesting, the depletion of this wild resource has been exacerbated. [0003] Morphological description of the peduncle tongue: ...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 兰进陈向东姚自奇曾念开
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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