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Dual variable domain immunoglobulins and uses thereof

A domain-binding protein technology for the prevention and/or treatment of acute and chronic inflammatory diseases, diagnosis, cancer and other diseases

Inactive Publication Date: 2011-12-28
ABBVIE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, this approach requires mutational modification of the immunoglobulin sequences in the constant regions, thus generating unnatural and unnatural forms of antibody sequences, which can lead to increased immunogenicity, poor in vivo stability, and undesired pharmacokinetics

Method used

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  • Dual variable domain immunoglobulins and uses thereof
  • Dual variable domain immunoglobulins and uses thereof
  • Dual variable domain immunoglobulins and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0498] Example 1: Design, construction and analysis of DVD-Ig

Embodiment 11

[0499] Example 1.1: Proteolytic cleavage of DVD-Ig with cleavable linkers

[0500] Enterokinase-cleaved DVD699 (denoted DVD699-C) and DVD701 (denoted DVD701-C) protein samples were generated as follows. Mix 180 μL of purified DVD-Ig at 1.1 mg / ml with 20 μL of 10X enterokinase cleavage buffer (500 mM Tris-HCl (pH 8.0), 10 mM CaCl 2 , 1% Tween-20) combination. 5 U / mg of EKMax (Invitrogen, Cat. No. E180-01) was added to the mixture, and the mixture was incubated at room temperature for 2 days. To confirm that all light chains were processed at the engineered site, samples (reduced and non-reduced) were run on SDS-PAGE before and after cleavage, and when the ~36 kDa band was no longer present in the reduced sample The reaction was considered complete when the ~24 kDa and ~12 kDa bands (corresponding to the expected cleaved LC fragments) were present instead. To confirm that the 12 kDa fragment remained bound to the cleaved DVD-Ig molecule, the mixture was additionally purified ...

Embodiment 12

[0508] Example 1.2: Assays for identification and characterization of parental antibodies and DVD-Ig

[0509] Unless otherwise indicated, the following assays were used throughout the Examples to identify and characterize the parental antibodies and DVD-Igs.

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Abstract

The present invention relates to engineered multivalent and multispecific binding proteins, methods of preparation, and in particular to their use in disease prevention, diagnosis and / or therapy.

Description

[0001] References to related applications [0002] This application is a non-provisional application claiming the benefit of U.S. Provisional Application Serial No. 61 / 200,877, filed December 4, 2008, and U.S. Provisional Application Serial No. 61 / 212,071, filed April 7, 2009, the contents of which are This is incorporated by reference. field of invention [0003] The present invention relates to multivalent and multispecific binding proteins, methods of preparation, and in particular their use in the diagnosis, prevention and / or treatment of acute and chronic inflammatory diseases, cancer and other diseases. Background of the invention [0004] Engineered proteins such as multispecific antibodies capable of binding two or more antigens are known in the art. Such multispecific binding proteins can be produced using cell fusion, chemical conjugation, or recombinant DNA techniques. [0005] Based on the somatic fusion of 2 different hybridoma cell lines, has been produced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/46A61K39/00
CPCC07K2317/24C07K2317/31C07K16/241C07K2317/64C07K16/468C07K16/244A61P1/00A61P1/16A61P11/00A61P11/06A61P13/12A61P17/00A61P17/06A61P19/00A61P19/02A61P25/00A61P25/14A61P25/16A61P25/18A61P25/24A61P25/28A61P27/02A61P29/00A61P31/14A61P31/18A61P31/20A61P33/00A61P35/00A61P37/00A61P37/02A61P37/04A61P37/08A61P5/00A61P7/00A61P9/00A61P9/10A61P3/10C07K16/46C12N15/62A61K39/395
Inventor C.G.雅各布吴辰冰K.A.沃特T.加于尔
Owner ABBVIE INC
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