Micro blood cell separation device and method for using same

A separation device, a technology for blood cells, applied in separation methods, dispersed particle separation, chemical instruments and methods, etc., can solve the problems of inability to meet high-throughput separation applications, low separation efficiency, etc., and achieve low cost, small cell damage, and high efficiency. high effect

Inactive Publication Date: 2012-01-04
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the above-mentioned separation methods all have the defect of low separation efficiency and cannot meet the application of large-throughput separation.

Method used

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  • Micro blood cell separation device and method for using same
  • Micro blood cell separation device and method for using same
  • Micro blood cell separation device and method for using same

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Experimental program
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Embodiment Construction

[0022] The present invention is described in further detail below in conjunction with accompanying drawing:

[0023] see image 3 , a miniature blood cell separation device, the separation device includes a signal generator, a sample pump, a separation chip and a cell collector after separation; the separation chip is provided with a separation chamber, the front end of the separation chamber is provided with an injection port, and the rear end is provided with There are two (or more) outlets, an electrode plate is arranged in the separation chamber, and an interdigitated electrode group is arranged on the electrode plate; the signal generator is connected to the interdigitated electrode group through a wire; the sample injection pump is connected to the The injection port is communicated; the outlet is connected with a cell collector after separation.

[0024] The channel plate is provided with a separation sample flow channel and a separation cavity, and is also provided wi...

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PUM

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Abstract

The invention discloses a micro blood cell separation device and a method for using the same. The separation device comprises a signal generator, a sample-adding pump, a separation chip and separated cell collectors; a separating cavity is arranged on the separation chip; the front end of the separation cavity is provided with an infusion opening; the rear end of the separation cavity is provided with two outlets; an electrode plate is arranged in the separation cavity; interdigital electrode groups are arranged on the electrode plate; the signal generator is connected with the interdigital electrode groups through conducting wires; the sample-adding pump is communicated with the infusion opening through a sample infusion pipe; and the outlets are connected onto the separated cell collectors. According to the invention, the separation is carried out by using the dielectrophoresis characteristic differences of blood cells in an electric field; and the continuous separation manner has the advantages of high efficiency, low cost, little cell damage, capability of large-flux separation and the like compared with the traditional cell separation method.

Description

Technical field: [0001] The invention relates to a miniature blood cell separation device and a using method thereof. A kind of blood cell separation using dielectrophoresis method, which can be used for continuous large-throughput separation. It has the advantages of simple structure, high separation efficiency, and small cell damage. It can be used in various fields such as medical diagnosis, biological reaction, and health quarantine. . Microseparation chip and method of use thereof. Background technique: [0002] Cell separation is an essential link in applications such as biochemical detection and medical diagnosis. Commonly used methods include filtration, adsorption-release, and magnetic separation. In the filtration method, the sample cells are passed through a filter, a filter screen, and a filter membrane of a specific size under pressure, and the separation process of cells of different sizes is realized by selecting the filter size. The adsorption release meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D57/02G01N1/38
Inventor 王小章王朝晖张群明陈豫刘佳
Owner XI AN JIAOTONG UNIV
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