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Real-time fluorescent polymerase chain reaction (PCR) identification method for cashmere and sheep wool

A real-time fluorescence, sheep wool technology, applied in biochemical equipment and methods, fluorescence/phosphorescence, microbial determination/inspection, etc., can solve problems such as the blank of molecular biology identification methods, and achieve the effect of good specificity and high sensitivity

Inactive Publication Date: 2012-01-04
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although my country is a big country for importing wool and exporting cashmere, there is still a blank in the study of molecular biological identification methods for cashmere and sheep wool

Method used

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  • Real-time fluorescent polymerase chain reaction (PCR) identification method for cashmere and sheep wool
  • Real-time fluorescent polymerase chain reaction (PCR) identification method for cashmere and sheep wool
  • Real-time fluorescent polymerase chain reaction (PCR) identification method for cashmere and sheep wool

Examples

Experimental program
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Embodiment Construction

[0016] 1 Test material

[0017] Rabbit fur, self-collected. Light green sweater, dark dark green sweater, gray sweater, beige sweater (label: 70% sheep wool and 30% cashmere), dark green sweater (label: 70% sheep wool and 30% cashmere ), gray plaid suit fabric, gray men’s suit fabric, blue sweater material (sign display: pure cashmere), black, white and gray three-color sweater material (sign display: 73.9% sheep wool, 11.6% cashmere and 14.5% camel hair) are all Collected by the textile laboratory of our unit. The purple cashmere sweater material and camel hair were presented by the representatives who participated in the "8th CU Series Fiber Fineness Meter Application Seminar and Eye Unification Exchange Meeting". The purple cashmere and raccoon fur were presented by researcher Zhang Yan from the National Textile Products Quality Supervision and Inspection Center of the National Academy of Textile Sciences. Among them, dark green sweater material, blue sweater material, a...

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Abstract

The invention provides a real-time fluorescent polymerase chain reaction (PCR) identification method for cashmere and sheep wool. The method comprises the following steps of: extracting deoxyribonucleic acid (DNA) of a sample; designing a primer and a probe for DNA sequences of mitochondria of goats and sheep; performing specific amplification on goat ingredients and sheep ingredients in the DNA respectively through a real-time fluorescent PCR; and identifying whether a sample to be detected contains the cashmere and the sheep wool or not according an amplification curve and a Ct value. The real-time fluorescent PCR identification method for the cashmere and the sheep wool is high in specificity and sensibility, and the requirements of daily detection of light textiles can be met.

Description

technical field [0001] The invention provides a real-time fluorescent PCR identification method for cashmere and sheep wool. Background technique [0002] Cashmere, commonly known as cashmere, is a rare and expensive animal fiber. Its fiber fineness is even, smooth, waxy, soft and delicate to the touch, strong in tension and elastic, known as "soft gold". Its cashmere products have extremely high quality and comfortable wearing performance, are loved by people, and the price is also high. In order to reduce costs, producers mix fine sheep wool or treated and modified sheep wool similar to cashmere into fabrics, so that counterfeit or reduced cashmere textiles appear on the market, which greatly damages the interests of consumers. Therefore, it is very important to effectively identify cashmere and sheep wool. [0003] Cashmere and sheep wool belong to the same protein fiber, and there is little difference in chemical properties, but there are some differences in scale st...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 邵碧英林志武陈文炳缪婷玉彭娟
Owner INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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