Selenium-containing MS (Murashige,T. and Skoog,F.) tissue culture medium

Abstract

The invention provides a selenium-containing MS (Murashige,T. and Skoog,F.) tissue culture medium which is used for increasing the disease resistance and pest resistance of grown tissue culture seedlings and the survival rate of the seedlings planted in soil. The selenium-containing MS tissue culture medium is characterized in that selenium is added in the existing MS tissue culture medium without selenium.

Description

technical field [0001] The invention relates to MS tissue culture matrix technology, in particular to a selenium-containing MS tissue culture medium. Background technique [0002] MS tissue culture matrix and induction matrix are the core technologies of tissue culture. The MS tissue culture medium in the prior art contains: carbon, hydrogen, oxygen, nitrogen, chlorine, potassium, calcium, phosphorus, sulfur, magnesium, iron, sodium, manganese, zinc, boron, copper, molybdenum, iodine, cobalt ten A compound or organic compound composed of several elements, usually its formula is as follows: 1. Major ingredients: potassium nitrate 1900 mg / L, ammonium nitrate 1650 mg / L, calcium chloride 440 mg / L, magnesium sulfate 370 mg / L, sulfurous acid Iron 27.8 mg / L, potassium dihydrogen phosphate 170 mg / L, sodium edetate 37.3 mg / L. 2. Trace ingredients: manganese sulfate 22.3 mg / L, zinc sulfate 8.6 mg / L, boric acid 6.2 mg / L, copper sulfate 0.025 mg / L, potassium iodide 0.83 mg / L, cobalt c...

AI Technical Summary

Problems solved by technology

[0004] However, the inventors found that in the practice of detoxification tissue culture, the tissue culture seedlings bred with MS tissue culture medium have poor disease resistance and insect pest resistance after growing up, and after being implanted in the soil, the survival rate is only 70%.