Selenium-containing MS (Murashige,T. and Skoog,F.) tissue culture medium

A technology of tissue culture medium and selenium element, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of poor resistance to disease and pests, and the survival rate is only 70%, and achieve strong resistance to pests and diseases Effect

Inactive Publication Date: 2012-02-08
金色种业有限公司
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  • Abstract
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Problems solved by technology

[0004] However, the inventors found that in the practice of detoxification tissue culture, the tissue culture seedlings bred with MS tissue culture medium have poor disease resistance and insect pest resistance after growing up, and after being implanted in the soil, the survival rate is only 70%.

Method used

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Embodiment Construction

[0016] MS tissue culture matrix and induction matrix are the core technologies of tissue culture. The prior art culture matrix contains: carbon, hydrogen, oxygen, nitrogen, chlorine, potassium, calcium, phosphorus, sulfur, magnesium, iron, sodium, manganese, zinc, Compounds or organic substances composed of nineteen elements such as boron, copper, molybdenum, iodine, and cobalt. Magnesium sulfate 370 mg / L, ferrous sulfate 27.8 mg / L, potassium dihydrogen phosphate 170 mg / L, sodium edetate 37.3 mg / L. 2. Trace ingredients: manganese sulfate 22.3 mg / L, zinc sulfate 8.6 mg / L, boric acid 6.2 mg / L, copper sulfate 0.025 mg / L, potassium iodide 0.83 mg / L, cobalt chloride 0.025 mg / L, sodium molybdate 0.25 mg / L. 3. Organic ingredients: adenine 5 mg / L, glycine 2 mg / L, inositol 100 mg / L, niacin 0.5 mg / L, vitamin B10.1 mg / L, vitamin B60.5 mg / L, indole Acetic acid 1 mg / L, 6-benzyl adenine 1 mg / L, sucrose 30000 mg / L, agar 8000 mg / L, H+1995 nmol. This formula lacks an essential element for p...

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Abstract

The invention provides a selenium-containing MS (Murashige,T. and Skoog,F.) tissue culture medium which is used for increasing the disease resistance and pest resistance of grown tissue culture seedlings and the survival rate of the seedlings planted in soil. The selenium-containing MS tissue culture medium is characterized in that selenium is added in the existing MS tissue culture medium without selenium.

Description

technical field [0001] The invention relates to MS tissue culture matrix technology, in particular to a selenium-containing MS tissue culture medium. Background technique [0002] MS tissue culture matrix and induction matrix are the core technologies of tissue culture. The MS tissue culture medium in the prior art contains: carbon, hydrogen, oxygen, nitrogen, chlorine, potassium, calcium, phosphorus, sulfur, magnesium, iron, sodium, manganese, zinc, boron, copper, molybdenum, iodine, cobalt ten A compound or organic compound composed of several elements, usually its formula is as follows: 1. Major ingredients: potassium nitrate 1900 mg / L, ammonium nitrate 1650 mg / L, calcium chloride 440 mg / L, magnesium sulfate 370 mg / L, sulfurous acid Iron 27.8 mg / L, potassium dihydrogen phosphate 170 mg / L, sodium edetate 37.3 mg / L. 2. Trace ingredients: manganese sulfate 22.3 mg / L, zinc sulfate 8.6 mg / L, boric acid 6.2 mg / L, copper sulfate 0.025 mg / L, potassium iodide 0.83 mg / L, cobalt c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李亚雄
Owner 金色种业有限公司
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