Drug and tumor whole-cell vaccine for treating or preventing tumor, and preparation methods and applications of drug and whole-cell vaccine

A whole-cell vaccine and tumor cell technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc.

Inactive Publication Date: 2012-02-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the use of IL-15 as a vaccine adjuvant

Method used

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  • Drug and tumor whole-cell vaccine for treating or preventing tumor, and preparation methods and applications of drug and whole-cell vaccine
  • Drug and tumor whole-cell vaccine for treating or preventing tumor, and preparation methods and applications of drug and whole-cell vaccine
  • Drug and tumor whole-cell vaccine for treating or preventing tumor, and preparation methods and applications of drug and whole-cell vaccine

Examples

Experimental program
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Effect test

preparation example Construction

[0120] 1. Preparation and method of IL-15 (amplification and purification of hIL-15 plasmid)

[0121] After the recombinant plasmid was confirmed to be correct by restriction enzyme digestion and sequencing, it was prepared in large quantities with the EndofreeTM plasma Giga kit of Qiagen Company. The method refers to the operation manual. The steps are as follows (the amount of the following buffers is suitable for 2.5L bacterial culture):

[0122] 1) Bacterial culture: the plasmid was inoculated in LB medium containing 100 μg / ml Amp, and cultured overnight at 37°C with shaking;

[0123] 2) Harvest bacteria: Centrifuge at 4000rpm / min for 30 minutes at 4°C, discard the supernatant, open the bottle and invert to drain all the supernatant, and weigh;

[0124] 3) Resuspension: Add 125ml Buffer P1 to the bacterial pellet, shake repeatedly, and blow until the bacteria are completely resuspended;

[0125] 4) Lysis: Add 125ml Buffer P2, gently and thoroughly invert the centrifuge ...

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Abstract

The invention relates to a drug and a tumor whole-cell vaccine for treating or preventing tumor, and preparation methods and applications of the drug and the whole-cell vaccine, and belongs to the fields of bioengineering and biological immunology. The invention provides a drug with good effect for treating or preventing tumor and a tumor whole-cell vaccine. The drug comprises the tumor whole-cell vaccine, a cation liposome, and recombinant plasmid for encoding interleukin-15 gene. The drug has the dual actions of tumor prevention immunization and anti-tumor immune treatment, and has durable action and obvious tumor prevention or treatment effect.

Description

technical field [0001] The invention relates to a drug for treating or preventing tumors, a tumor whole-cell vaccine (Whole-cell vaccine; Whole cell vaccine) and a preparation method and use thereof, belonging to the fields of bioengineering and bioimmunology. Background technique [0002] Malignant tumors have a variety of immune escape mechanisms, including low tumor cell antigen specificity and / or tumor antigen destruction, or lack of expression of some immune cofactors, etc., making the clinical treatment effect of existing tumor vaccines poor. In addition, in the advanced stage of tumors, tumor cells are highly heterogeneous and prone to mutations to escape the supervision of the immune system. Mutations lead to the disappearance of tumor antigen phenotypes, which in turn reduces the effect of immunotherapy targeting this antigen. In order to optimize the efficacy of tumor vaccines, cytokines (such as IL-12, GM-CSF) are often used as adjuvants to enhance the immune effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/39A61P35/00
Inventor 周良学吴扬郭刚仝爱平魏于全陈曦
Owner SICHUAN UNIV
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