Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of astragaloside in preparation of drugs for promoting epithelization in wound healing

A technology for wound healing and astragaloside IV, which is applied in the application field of astragaloside IV in the preparation of a drug for promoting wound healing and epithelization, and achieves the effects of broad industrialization prospects, low price, and speeding up the process of epithelization.

Inactive Publication Date: 2012-02-15
YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of astragaloside IV in the preparation of drugs for promoting epithelialization of wound healing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of astragaloside in preparation of drugs for promoting epithelization in wound healing
  • Application of astragaloside in preparation of drugs for promoting epithelization in wound healing
  • Application of astragaloside in preparation of drugs for promoting epithelization in wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] LiCL mimics activate the canonical Wnt signaling pathway

[0038] 1. MTS / PMS method to detect LiCL simulated activation of canonical Wnt signaling pathway and its effect on keratinocyte proliferation

[0039] MTS / PMS method was used to detect the effect of LiCL on the proliferation of keratinocytes. The results showed that the mouse keratinocytes were treated with different concentrations of LiCL from 0.5mM to 40mM for 24h, 48h and 72h, and LiCL started when the concentration was greater than 5mM. Significantly inhibited keratinocyte growth (see Figure 9 ). The IC50 of LiCL at 24h, 48h and 72h was 25.85±5.03, 16.16±0.95, 12.35±1.38mM respectively. Therefore, in this experiment, 20mM concentration was used to culture for 48h to carry out subsequent research on AS-IV.

[0040] 2. BrDU staining method to determine the effect of LiCL on the proliferation of keratinocytes

[0041] The principle of BrDU staining is: in the S phase of the cell cycle, the BrdU incubated ...

Embodiment 2

[0049] AS-IV improves the proliferation inhibition of keratinocytes by LiCL

[0050] We observed the effect of AS-IV on keratinocyte proliferation under mimicked activation of the canonical Wnt signaling pathway by LiCL. The result is as Figure 5 As shown, after the intervention of 20mM LiCL, the cell proliferation was significantly inhibited, and different concentrations of AS-IV had a significant correction effect on this inhibition, which could improve the inhibition of the cells, and the effect was the strongest at 72 hours. Depend on Figure 6 It can be seen that as the concentration of AS-IV increases, the corrective effect is more pronounced.

Embodiment 3

[0052] AS-IV improves the migration inhibition of keratinocytes by LiCL

[0053] In order to observe the effect of AS-IV on the migration function of keratinocytes in vitro, we used LiCL to simulate the activation of the canonical Wnt signaling pathway, and then confirmed it by scratching experiments. The result shows: if Figure 7 As shown in A, the migratory ability of keratinocytes activated by LiCL was significantly weakened, and scratch damage took longer to repair, while the control group added with epidermal growth factor (EGF) or AS-IV had an inhibitory effect on cell migration can be improved to a certain extent. like Figure 7 As shown in B, the immunofluorescence of LiCL-treated wound keratinocytes showed nuclear expression of β-catenine, and at the edge of the artificial scratch after LiCL intervention, keratinocyte migration was blocked at the place where β-catenine nuclear expression was most obvious, while AS- IV reduces this effect, and cell migration begi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of astragaloside in preparation of drugs for promoting epithelization in wound healing. The astragaloside can correct the inhibition effect on the proliferation and migration of keratinocyte under the condition of imitating and activating a classical Wnt signaling channel with lithium chloride, thus the astragaloside has promotion action on the epithelization stage in wound healing process and can be used for preparation of the drugs for treating chronic skin ulcer or other skin wound surfaces. The invention has the advantages that: the new application of the astragaloside in pharmacy is developed, and a new application field is developed; the astragaloside can promote the proliferation and migration of keratinocyte, namely accelerate the epithelization course in wound healing process, therefore, the astragaloside has the effect of promoting wound healing and an important clinical basis is provided for prevention and treatment of chronic skin ulcer; and the astragaloside has wide raw material sources, low price, s and broad industrial prospects, and the preparation process of the astragaloside is simple. The astragaloside is suitable for wide popularization and use.

Description

technical field [0001] The invention relates to a new application of astragaloside IV in the field of pharmacy, in particular to the application of astragaloside IV in the preparation of medicines for promoting epithelialization of wound healing. Background technique [0002] The traditional Chinese medicine Astragalus has the effects of nourishing, detoxifying, and promoting muscle growth. It is recorded in "Compendium of Materia Medica" that it has the functions of evacuating pus and relieving pain, promoting blood circulation and promoting granulation, and internally supporting genital gangrene. Astragaloside IV is one of the main active ingredients of the traditional Chinese medicine Astragalus, and its structural formula is: [0003] [0004] The molecular formula of this compound is: C 41 h 68 o 14 , molecular weight 784.98, 20%-30% of astragaloside IV is yellow powder, 90% of astragaloside IV is white powder, 98% of astragaloside IV is colorless needle crystal,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/7048A61P17/02
Inventor 李福伦李斌李欣王一飞范斌徐蓉陈洁
Owner YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com