Method for planting king oyster mushroom by using grape branch
A technology of king oyster mushrooms and branches is applied in the directions of botanical equipment and methods, application, horticulture, etc., can solve the problems of shortage of raw materials, environmental pollution, edible fungi, low utilization rate, etc., to avoid production technology and environmental pollution, save money Resources, the effect of simplifying the degradation pathway for reuse
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[0026] 1. Cultivation of PDA first-class material - mother species (first-class species)
[0027] 1. Prepare PDA enriched medium: potato 200g / L, glucose 10g / L, sucrose 10g / L, peptone 1g / L, agar 12g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L, pH7.0
[0028] 2. Sterilize for 30 minutes, make a slope
[0029] 3. Inoculate and culture at 23°C for 7-10 days in the dark
[0030] two. Propagation of mother species - original species (secondary species)
[0031] 1. Preparation of wheat grain medium: boil the wheat for half an hour, add 10g / kg of glucose, mix well, let it cool down and pack it into bags.
[0032] 2. Sterilize for 30 minutes, then insert the first-grade species after cooling
[0033] 3. Cultivate at constant temperature and dark at 23°C for about 20 days
[0034] 3. Propagation of original species - cultivated species
[0035] 1. Prepare cultivation material: Mix 68.03 grams of grape branch powder, 20.41 grams of bran, 3.40 grams of corn flour, and 8.16 grams of soybean ...
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