Method for measuring content of metallic ion in metal protein

A determination method and metalloprotein technology, which is applied in the preparation of test samples and the measurement of color/spectral properties, etc., can solve the problems of low repeatability and accuracy, increase the development cost of scientific research projects, and vary widely in analysis results. The effect of improving precision and reproducibility, shortening nebulization processing time, and improving analytical efficiency
CN102393365AActive Publication Date: 2012-03-28BEIJING TIDE PHARMA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING TIDE PHARMA
Publication Date
2012-03-28

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Abstract

The invention discloses a method for measuring the content of a metallic ion in a metal protein, which comprises the following steps of: pretreating a sample, preparing or treating the sample with an acid material and enabling the pH value of a sample solution to be smaller than 5; and then measuring by a metallic element measuring spectrum (an atomic absorption spectroscopy or an atomic emission spectrometer). According to the method, the problems of low accuracy, poor precision, poor reproducibility, poor specificity and the like in the method for measuring the content of the metallic ion in the metal protein are solved; the detection time can be shortened; the analysis efficiency is improved and the analysis cost is reduced.
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Description

technical field

[0001] The invention relates to a method for determining the content of metal ions in metalloproteins, in particular to a method for accurately quantifying the content of metal ions in metalloproteins through sample pretreatment. Background technique

[0002] Metalloproteins are formed by binding proteins and metal ions. Most of the metal ions are only connected to the protein; a few are connected to a smaller molecule in addition to the protein, such as iron (II) in hemoglobin, in addition to being connected to the protein, it is also connected to the porphyrin ring. Metalloproteins have important physiological functions. For example, hemoglobin is necessary for transporting oxygen; ceruloplasmin can catalyze the oxidation of iron (II) to facilitate the combination of iron (III) and protein to form transferrin, which is used to transport iron, etc. . The content of metal ions in metalloproteins plays a vital role in the function of organisms. Recent studie...

Claims

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