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Preparation method for composite continuous cropping resistant agent specially used for strawberries

An anti-stubble agent, strawberry technology, applied in the application, fertilizer mixture, fertilization device and other directions, can solve the problems of long production cycle, no spore formation, and mycelium effect is not as good as spores, etc., to reduce yield, sugar-acid ratio, etc. The effect of improving quality and improving micro-ecological environment

Active Publication Date: 2012-04-25
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two methods of producing antibiotic fertilizer with Streptomyces luteus, solid and liquid fermentation. The production cycle of solid fermentation is long and the product quality is unstable; liquid fermentation products exist in the form of mycelium and do not form spores. On the one hand, it is not conducive to Preservation and storage, the shelf life of the product is short, on the other hand, the effect of mycelium in application is not as good as that of spores

Method used

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  • Preparation method for composite continuous cropping resistant agent specially used for strawberries

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Effect test

Embodiment 1

[0045] The preparation method of the special compound anti-stubble agent for strawberries comprises the following process steps:

[0046] A, the preparation of Streptomyces flavinus antibiotic agent

[0047] A1, the preparation of liquid seed

[0048] Inoculate the original strains into the sterilized liquid medium for shaker culture to make liquid seeds. The culture conditions are 180 rpm, 28°C, and 24 hours; add about 5 ml of sterile Water in the test tube strains, use an inoculation needle to scrape up the bacterial lawn to make a bacterial suspension, and inoculate 3-5 triangular flasks on each inclined plane;

[0049] Above-mentioned liquid culture medium is made up of the raw material of following mass percentage:

[0050] Corn flour 1.8%, bean cake powder 2%, starch 0.8%, and the balance is water;

[0051] The original strain was selected from the strain Streptomyces flavinus ( Streptomyces micrcflav ), whose deposit number is CGMCC4.891;

[0052] A2, solid ferment...

Embodiment 2

[0069] The preparation method of the special compound anti-stubble agent for strawberries comprises the following process steps:

[0070] A, the preparation of Streptomyces flavinus antibiotic agent

[0071] A1, the preparation of liquid seed

[0072] Inoculate the original strains into the sterilized liquid medium for shaker culture to make liquid seeds. The culture conditions are 180 rpm, 30°C and 24 hours of culture period; add about 5 ml of sterile Water in the test tube strains, use an inoculation needle to scrape up the bacterial lawn to make a bacterial suspension, and inoculate 3-5 triangular flasks on each inclined plane;

[0073] Above-mentioned liquid culture medium is made up of the raw material of following mass percentage:

[0074] Corn flour 2%, bean cake powder 2.5%, starch 1.2%, and the balance is water;

[0075] The original strain was selected from the strain Streptomyces flavinus ( Streptomyces micrcflav ), whose deposit number is CGMCC4.891;

[0076] ...

Embodiment 3

[0093] The preparation method of the special compound anti-stubble agent for strawberries comprises the following process steps:

[0094] A, the preparation of Streptomyces flavinus antibiotic agent

[0095] A1, the preparation of liquid seed

[0096] Inoculate the original strains into the sterilized liquid medium for shaker culture to make liquid seeds. The culture conditions are 180 rpm, 29°C, and 24 hours of culture time; add about 5 ml of sterile Water in the test tube strains, use an inoculation needle to scrape up the bacterial lawn to make a bacterial suspension, and inoculate 3-5 triangular flasks on each inclined plane;

[0097] Above-mentioned liquid culture medium is made up of the raw material of following mass percentage:

[0098] Corn flour 2%, bean cake flour 2.3%, starch 1%, and the balance is water;

[0099] The original strain was selected from the strain Streptomyces flavinus ( Streptomyces micrcflav ), whose deposit number is CGMCC4.891;

[0100] A2, ...

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Abstract

The invention especially relates to a preparation method for a composite continuous cropping resistant agent specially used for strawberries, which belongs to preparation of microbial compound agents. The method comprises the steps of preparation of a Streptomyces microflavus antibiotic microbial agent, preparation of a trace element additive and preparation of the composite continuous cropping resistant agent specially used for strawberries by compounding microbial fertilizer biological potassium-phosphorous composite fertilizer, the Streptomyces microflavus antibiotic microbial agent and the trace element additive in propotion. The invention enables the problems of high cost, high toxicity and the like caused by utilization of the chemical method of fumigating for controlling replant disease of strawberries in the prior art to be overcome and allows microecological environment of soil to be improved and the nutrient elements of phosphorous and potassium needed for growth of strawberries to be provided. Application of the special continuous cropping resistant agent prepared in the invention in planting of strawberries enables yield increase to be effectively realized and soluble solid, sugar content, sugar-acid ratio and other quality of strawberries to be improved.

Description

technical field [0001] The invention belongs to the preparation of microbial compound preparations, in particular to a preparation method of a special compound anti-stubble agent for strawberries. Background technique [0002] Strawberry berries are bright red, fragrant and juicy, sweet and sour, and rich in nutrition. They are regarded as treasures among fruits. At present, there are nearly 40,000 hectares of strawberry cultivated in the country, with an output of more than 200,000 tons. Strawberry cropping disease is an important problem in strawberry production. Research at home and abroad shows that the main cause of crop cropping is the lack of soil nutrients and soil-borne diseases. Strawberry cropping in the same field for 2 years generally reduces yield by 10-15% , The production will be reduced by 20%-25% for 3 years, and the production will be reduced by more than 40% for more than 4 years. In severe cases, the harvest may even be lost. [0003] In the process of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 秦艳梅唐兆宏李军马清河赵从波陈文杰章淑艳魏亚新李宾古述江
Owner 河北省微生物研究所有限公司
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