Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora

A technique for culturing immature embryos and a culture medium, which is applied in the field of medium formulations for culturing immature embryos of Paeonia suffruticosa, can solve the problems of poor development of embryos of Paeonia japonicas, failure of normal sowing and emergence, and the like, so as to overcome technical obstacles and shorten the breeding cycle. , the effect of increasing growth rate

Inactive Publication Date: 2012-05-02
YANGZHOU UNIV
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Problems solved by technology

[0005] In order to solve the technical problem that the double-flowered peony can not be sown and sprouted normally due to poor embryonic development, the present invention provides

Method used

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  • Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora
  • Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora
  • Culture medium and culture method for cultivating immature embryo of double-petal paeonia lactiflora

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Example Embodiment

[0026] Example 1:

[0027] To carry out this research work with the double-petaled peony variety "Hongyan Zhenghui" as the test material:

[0028] (1) Material: Collect the pods of "Hongyan Zhenghui" whose peel has begun to turn color in early July, soak in washing liquid for 10 minutes, rinse the washing liquid with tap water, soak in 1% for 20 minutes, then rinse with tap water and peel off the fruit. Pod, take out the seeds, first use 75% alcohol to surface sterilize for 30 s, then use 0.1% mercury liters for 8 minutes, and rinse with sterile water 5-6 times. Put it on sterile filter paper, cut the seeds with a scalpel, and pick out the seed embryos with a dissecting needle.

[0029] (2) Medium formula:

[0030] ① Inoculate the seed embryos in the following three media for inducing adventitious buds: 1) Modified MS + TDZ 0.5 mg / L, sucrose 100g·L -1 , Agar 6g·L -1 , PH is 5.8; 2), modified MS + TDZ 1.0 mg / L, sucrose 100g·L -1 , Agar 6g·L -1 , 3), modified MS + TDZ 2.0 mg / L, sucro...

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Abstract

The invention specifically relates to a culture medium prescription for cultivating immature embryo of double-petal paeonia lactiflora and a method for cultivating the seedlings of the paeonia lactiflora by using the culture medium. The culture medium comprises adventitious bud induction culture medium, adventitious bud strengthening culture medium and rooting culture medium; and the culture medium is formed by adding TDZ, cane sugar and agar based on improved MS or 1/2 improved MS. The culture method comprises the following steps: selecting the seedling embryo; preparing the culture medium; and inoculating to cultivate, wherein the step of inoculating to cultivate is the step of adopting the three culture mediums to induce the adventitious bud, strengthen the adventitious bud and root. Because of the invention, the seedling rate of the immature embryo reaches over 60%, and the technical obstacle that the double-petal paeonia lactiflora cannot establish the seedlings caused by the dysplasia of the seedling embryo. Each germination seedling embryo can establish 3-8 adventitious buds and 5-10 fleshy roots, so the effect that the seedlings of the normal seed differentiate the buds after three years is realized, the growth speed of the seedlings of the paeonia lactiflora is improved, the hybrid seedlings of the paeonia lactiflora flowers at early stage, and the breeding period of the paeonia lactiflora is shortened.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a medium formula for cultivating immature embryos of peony double petals and a method for cultivating peony seedlings using the medium. Background technique [0002] The existing excellent ornamental varieties of peony in my country are mainly obtained through natural seed selection, and the books about the introduction of peony varieties do not indicate the source of their parents. Although many scientific research units have carried out hybrid breeding of peony through the selection and mating of paeoniae officinalis parents, the progress has been slow for many years. The reason is that the peony ( Paeonia lactiflora Pall.) The most important double-petal trait in the ornamental quality is formed by the petalization variation after the degeneration of the pistil or stamen. Therefore, it is difficult to fertilize and set fruit when using double-petal varieties of peony with ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陶俊薛银芳赵大球葛金涛韩晨霞
Owner YANGZHOU UNIV
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