Laser microdissection apparatus and method

Abstract

The invention provides a laser microdissection device and method, the laser microdissection device includes a laser and an objective lens; the micromirror array group includes a plurality of microreflectors arranged in an array; the output light of the laser passes through the The reflection of the micromirror array group is directed to the objective lens; the drawing unit is suitable for the operator to draw a cutting curve; the calculation unit obtains each of the micromirror array groups corresponding to any point in the cutting curve according to the drawn cutting curve The inclination angles of the micro-mirrors relative to the output light are transmitted to the control unit; the control unit adjusts each micro-mirror according to the received inclination angles. The invention has the advantages of simple structure, accurate cutting and the like.

Description

technical field [0001] The invention relates to the field of biology, in particular to a laser microdissection device and method. Background technique [0002] At present, laser microdissection generally adopts the following two methods: [0003] 1. DMD realizes closed cutting curve, DMD adjustment angle is consistent, please refer to Chinese patent CN107186364A; the disadvantage of this solution is: [0004] Only parallel light can be reflected, and only one point can be obtained through the objective lens, which is not a curve; at the same time, errors such as aberrations generated after the optical path passes through the objective lens are not considered. [0005] 2. Use the transmission components to move the light source or scan the galvanometer to change the direction of light travel. The disadvantages of this scheme are: [0006] The transmission components are bulky, with low precision and high energy consumption. [0007] At present, there is a micromirror arra...

AI Technical Summary

Problems solved by technology

[0009] 1. The cutting of any curve cannot be realized, that is, the corresponding relationship between the cutting curve and the action of the micromirror array group has not been established;

[0010] 2. The micromirror array group adjusts the direction of the output light, and combines with the objective lens to converge the cutting light on the cutting object, and then uses the angle adjustment of the micromirror array to change the direction of the output light, so that the converging point of the cutting light sweeps over the cutting object At different positions corresponding to the cutting curve, it takes a long time, troublesome manipulation, and large errors;

[0011] 3. The application of the micromirror array group increases errors such as aberration and chromatic aberration;

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