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Arthrobacter CW9 and its purpose

A technology of Arthrobacter and its application, which is applied in the field of Arthrobacter CW9 and its application, and can solve the problems of the inhibitory effect of pathogenic bacteria on crustaceans in difficult seawater

Active Publication Date: 2013-08-28
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant seawater crustacean larvae probiotic product available in China at present.
Microbial products such as photosynthetic bacteria and bacillus are mainly used to regulate the water quality of aquaculture water, and it is difficult to have a specific inhibitory effect on seawater crustacean pathogenic bacteria

Method used

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  • Arthrobacter CW9 and its purpose
  • Arthrobacter CW9 and its purpose
  • Arthrobacter CW9 and its purpose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1. An Arthrobacter CW9 ( Arthrobacter sp. CW9) CGMCC No. 4197.

Embodiment 2

[0071] Example 2. Arthrobacter CW9 described in embodiment 1 ( Arthrobacter sp. CW9) In CGMCC No. 4197, the strain characteristics of Arthrobacter CW9 described are: the strain is a gram-positive bacterium, without spores, without capsules, and immobile; the colonies on the 2216E plate are yellow, round, and protruding in the middle , smooth and moist; most of the cells in the quiescent phase are spherical, φ0.5-1μm, and a few are short rod-shaped φ0.2-0.4??m×1.4-2.0??m; all of them are rod-shaped in the vigorous growth phase, with round ends, It is often arranged in a V shape; it has the typical characteristics of Arthrobacter, that is, Gram-positive in the stage of cocciform bacteria, weakly positive or even negative in the stage of rod-shaped bacteria; aerobic, and the growth temperature ranges from 0°C to 28°C, the optimum growth The temperature is 26°C, the pH range for growth is 5.5-11.0, and the optimum pH is 7.6; the NaCl-resistant concentration is 10%, the optimum...

Embodiment 3

[0072] Example 3. Arthrobacter CW9 described in embodiment 1 or 2 ( Arthrobacter sp. CW9) In CGMCC No. 4197, the fermentation medium of Arthrobacter CW9 is: glucose 7g / L, ammonium sulfate 6g / L, yeast extract 1.5g / L, NaCl 1g / L, pH7.6; the fermentation conditions are : The inoculum size is 5%, 20°C, 175r / min, and the culture period is 21h.

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Abstract

The invention relates to Arthrobacter CW9 (Arthrobacter sp. CW9) with CGMCC of No.4197. The bacterial strain is Gram positive bacteria without spore, capsule and motion. The colony on a 2216E flat possesses the characteristics of yellow, circle, intermediate protrusion, smoothness and moisture; The aerobic strain possesses optimum growth temperature of 26 DEG C and the optimum pH value of 7.6; the concentration for NaCl resistance is 10%, the optimum salinity is 1%, the growth can not generated without NaCl. The Arthrobacter CW9 can be used for raising the metamorphosis rate and survival rateof seawater crustaceans larvae. The bacterial strain screening method of the present invention is concise and high efficient, the Arthrobacter possesses probiotic function on the crustaceans larvae, the production method as well as the storage and transportation are simple, the application method is also simple; the Arthrobacter is employed only in crustaceans animal zoea one-stage, so that the metamorphosis rate of the post-larvae can be enhanced and stabilized, and the sprout seed output can be greatly increased.

Description

technical field [0001] The invention relates to an aquatic probiotic Arthrobacter CW9 ( Arthrobacter sp. CW9) CGMCC No. 4197 (preserved in the General Microorganism Center CGMCC of China Microbiological Culture Collection Management Committee on October 8, 2010, and the preservation number is CGMCC No. 4197); the present invention also relates to the use of this strain. Background technique [0002] In recent years, due to the aggravation of self-pollution of the aquaculture environment and the increasingly serious pollution of the natural environment, the living environment of farmed animals has continued to deteriorate, the number of pathogenic microorganisms has increased, and the speed of transmission has accelerated, causing major losses to the aquaculture industry. At the same time, the residues of certain prohibited chemicals used to prevent and treat diseases in animals have greatly affected the quality of products. However, due to the residues of prohibited dru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01K61/00C12R1/06
CPCY02A40/81
Inventor 朱明莫照兰阎斌伦夏振强陈静
Owner HUAIHAI INST OF TECH
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