Content determination method for anabasine
A technology of star anise base and star anise base, applied in the field of content determination of star anise base
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[0049] Instruments and reagents used: Shimadzu LC-2010A high performance liquid chromatography; KQ-250DB numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); Sartorius CP225D electronic balance (Beijing Sartorius Instrument System Co., Ltd.); BL610 Type electronic balance (Beijing Sartorius Instrument System Co., Ltd.); chromatographic column adopts Alltech Apollo C18 (4.6mm × 250mm, 5 μm); star anise base reference substance (purchased from Toronto Research chemicals lnc, batch number: A637175, used for content determination ); star anise maple (self-made, batch numbers: 20090501, 20090601, 20090602, 20090603); acetonitrile is chromatographically pure; other reagents are analytically pure; water is ultrapure filtered water.
[0050] Chromatographic conditions: Octadecylsilane bonded silica gel as filler, with acetonitrile:buffer (buffer: 0.2g sodium heptanesulfonate, 3.5g potassium dihydrogen phosphate to 1000mL)=9:91 (volume ratio ) is the mobile p...
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