Plasmodium antibody colloidal gold detection kit and preparation method thereof

A detection kit and colloidal gold technology, applied in the direction of measuring devices, diseases resistant to media transmission, instruments, etc., can solve the problems of low work efficiency, reduced accuracy of film reading, increased complexity, etc., and achieve strong specificity

Active Publication Date: 2012-07-18
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses an ordinary biological microscope for manually shifting and focusing, and the microscope inspectors often suffer from eye fatigue, resulting in reduced reading accuracy and low work efficiency.
The development of immunochromatography technolo

Method used

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  • Plasmodium antibody colloidal gold detection kit and preparation method thereof
  • Plasmodium antibody colloidal gold detection kit and preparation method thereof
  • Plasmodium antibody colloidal gold detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0057] Example 1 Preparation of kit

[0058] 1. Material

[0059] 1.1 30 Balb / c mice, 6-8 weeks old, raised by the company;

[0060] 1.2 Reagent source: Goat anti-mouse IgG polyclonal antibody, HRP-II-pLDH recombinant protein: purchased from MAXMED LABORATORIES INC., anti-HRP-II monoclonal antibody and anti-pLDH monoclonal antibody ( It can be prepared by referring to existing literature reports, such as Hao Wenbo, Li Ming, etc. Preparation and identification of recombinant HRP-II monoclonal antibodies against Plasmodium falciparum[J]. Chinese Journal of Parasitic Disease Control, 2000, 13(3): 165- 168; Wang Junyun, Bao Yifang, Yang Yuetao, Tang Linhua, Preparation of Plasmodium falciparum lactate dehydrogenase specific monoclonal antibody. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 4 etc.)

[0061] Nitrocellulose film, glass fiber paper: purchased from SARTORIUS, Germany

[0062] 2 method

[0063] 2.1 Preparation of colloidal gold: prepare colloidal gold with a part...

Example Embodiment

[0127] Example 2 Sensitivity Test of Plasmodium Antibody Colloidal Gold Colloidal Gold Detection Kit

[0128] Detection method:

[0129] 1. Take the Plasmodium antibody colloidal gold detection kit prepared in Example 1, and place the kit on a water platform.

[0130] 2. Use the sample pipette to suck the sample, and then drop 2 drops (about 50-80μl) of the sample into the sample hole of the kit, and then add 2 drops of normal saline. Use different pipettes for each different sample.

[0131] 3. Observation result: Interpret the result 20 minutes after adding the sample.

[0132] Test sample:

[0133] Configure HRP-II concentration of 10ng / ml, 15ng / ml, 20ng / ml, 25ng / ml quality control reference materials as samples.

[0134] Configure quality control reference materials with pLDH concentrations of 5ng / ml, 10ng / ml, 15ng / ml, and 20ng / ml as samples.

[0135] Test results:

[0136] HRP-II concentration (ng / ml)

[0137] pLDH concentration (ng / ml)

[0138] The results confirmed that the pr...

Example Embodiment

[0139] Example 4 Specificity Experiment of Plasmodium Antibody Colloidal Gold Detection Kit

[0140] Take the test kit prepared in Example 1 and add samples to test according to the following table. The test results are as follows:

[0141] Unit: ng / ml

[0142] Sample

[0143] This shows that the kit has good specificity.

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Abstract

The invention relates to a plasmodium antibody colloidal gold detection kit and a preparation method thereof. The plasmodium antibody colloidal gold detection kit comprises reagent strips, namely a piece of sample filter paper, an immune colloidal gold paper sheet, an immune nitrocellulose membrane and a piece of water absorption paper sequentially from a sample injection end, wherein an HRPII-pLDH bispecific monoclonal antibody marked by colloidal gold is wrapped on the immune colloidal gold paper sheet; an anti-HRPII specific monoclonal antibody and an anti-pLDH specific monoclonal antibody are sprayed in a detection region (T) of the nitrocellulose membrane; and goat-anti-mouse immune globulin (IgG) is sprayed in a quality control region (C) of the nitrocellulose membrane. The plasmodium antibody colloidal gold detection kit can detect Malaria sensitively, specifically and quickly.

Description

technical field [0001] The invention relates to a detection kit, in particular to a malaria parasite antibody colloidal gold detection kit and its preparation. Background technique [0002] Malaria (Malaria) is caused by the transmission of Plasmodium by the bite of a mosquito in the tropics and subtropics. Different Plasmodium causes Plasmodium vivax, Plasmodium malaria, Plasmodium falciparum and Plasmodium ovale respectively. The main manifestations of this disease are periodic attacks, chills, fever, and sweating all over the body. After repeated attacks for a long time, anemia and splenomegaly can be caused. Malaria is more common in summer and autumn, and can occur throughout the year in tropical and subtropical regions, and it is easy to spread. Malaria is still one of the most serious parasitic diseases threatening and endangering people's health in Asia and Africa. At present, there are still 120 million malaria patients in the world, and about 300 million carriers...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
CPCY02A50/30
Inventor 张国华
Owner SHANGHAI CHEMTRON BIOTECH
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