Plasmodium antibody colloidal gold detection kit and preparation method thereof
A detection kit and colloidal gold technology, applied in the direction of measuring devices, diseases resistant to media transmission, instruments, etc., can solve the problems of low work efficiency, reduced accuracy of film reading, increased complexity, etc., and achieve strong specificity
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[0057] Example 1 Preparation of kit
[0058] 1. Material
[0059] 1.1 30 Balb / c mice, 6-8 weeks old, raised by the company;
[0060] 1.2 Reagent source: Goat anti-mouse IgG polyclonal antibody, HRP-II-pLDH recombinant protein: purchased from MAXMED LABORATORIES INC., anti-HRP-II monoclonal antibody and anti-pLDH monoclonal antibody ( It can be prepared by referring to existing literature reports, such as Hao Wenbo, Li Ming, etc. Preparation and identification of recombinant HRP-II monoclonal antibodies against Plasmodium falciparum[J]. Chinese Journal of Parasitic Disease Control, 2000, 13(3): 165- 168; Wang Junyun, Bao Yifang, Yang Yuetao, Tang Linhua, Preparation of Plasmodium falciparum lactate dehydrogenase specific monoclonal antibody. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 4 etc.)
[0061] Nitrocellulose film, glass fiber paper: purchased from SARTORIUS, Germany
[0062] 2 method
[0063] 2.1 Preparation of colloidal gold: prepare colloidal gold with a part...
Example Embodiment
[0127] Example 2 Sensitivity Test of Plasmodium Antibody Colloidal Gold Colloidal Gold Detection Kit
[0128] Detection method:
[0129] 1. Take the Plasmodium antibody colloidal gold detection kit prepared in Example 1, and place the kit on a water platform.
[0130] 2. Use the sample pipette to suck the sample, and then drop 2 drops (about 50-80μl) of the sample into the sample hole of the kit, and then add 2 drops of normal saline. Use different pipettes for each different sample.
[0131] 3. Observation result: Interpret the result 20 minutes after adding the sample.
[0132] Test sample:
[0133] Configure HRP-II concentration of 10ng / ml, 15ng / ml, 20ng / ml, 25ng / ml quality control reference materials as samples.
[0134] Configure quality control reference materials with pLDH concentrations of 5ng / ml, 10ng / ml, 15ng / ml, and 20ng / ml as samples.
[0135] Test results:
[0136] HRP-II concentration (ng / ml)
[0137] pLDH concentration (ng / ml)
[0138] The results confirmed that the pr...
Example Embodiment
[0139] Example 4 Specificity Experiment of Plasmodium Antibody Colloidal Gold Detection Kit
[0140] Take the test kit prepared in Example 1 and add samples to test according to the following table. The test results are as follows:
[0141] Unit: ng / ml
[0142] Sample
[0143] This shows that the kit has good specificity.
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