Method for detecting content of fructooligosaccharide in infant formula milk rice flour

Abstract

The invention relates to a method for detecting content of fructooligosaccharide in infant formula milk rice flour. The method comprises the following steps: accurately weighting a sample; placing the sample into a volumetric flask; adding hot water for dissolving; mixing uniformly; ultrasonically shaking; cooling to room temperature; accurately adding a potassium ferrocyanide solution and a zinc acetate solution, shaking and uniformly mixing, adding water for achieving a constant volume, and uniformly shaking; standing; filtering by using filter paper; accurately transferring supernate to the volumetric flask until constant volume is achieved by using acetonitrile; shaking and uniformly mixing; filtering through a 0.45 micron organic filter film; performing HPLC (High Performance Liquid Chromatography) measuring and quantifying according to an external standard method, namely, firstly, preparing a standard working solution and respectively injecting the standard working solution into the HPLC, measuring a corresponding peak area and drawing a standard curve, then injecting the prepared sample solution into the HPLC, measuring the corresponding peak area and quantifying the sample on the basis of the standard curve, thereby obtaining the concentration of the sample solution; and calculating the percentage of the content of fructooligosaccharide according to a formula. The method has the advantages of convenience, high speed, accuracy and the like.

Description

technical field [0001] The invention relates to a method for detecting the content of fructooligosaccharides in formula milk rice flour for infants and young children. Background technique [0002] Fructose-oligosaccharide is a natural active substance, also known as fructo-oligosaccharides, which is composed of 1 to 3 fructosyl groups combined with fructosyl groups in sucrose through β (2-1) glycosidic bonds. A mixture of fructotetraose and sucrose pentaose. It can significantly improve the proportion of microbial populations in the intestine, and has a selective proliferation effect on beneficial bacteria in the intestine (such as bifidobacteria, lactobacilli, etc.). It can inhibit the growth of harmful bacteria, reduce the formation and accumulation of toxic substances in the intestine, promote intestinal peristalsis, and effectively prevent constipation, diarrhea and the occurrence of pathological bowel cancer. At the same time, fructo-oligosaccharides are fermented by...

AI Technical Summary

Problems solved by technology

This method is very simple in sample processing, and it is directly dissolved in ultrapure water to a constant volume, which is not suitable for infant formula rice flour with a relatively complex matrix.

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