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Equivalent and quantitative test method of white spot syndrome virus resistance of prawns

A technology of white spot syndrome and testing method, which is applied in the field of selection and breeding of disease-resistant varieties of prawns, to achieve the effects of reliable data, guaranteed identity, and easy operation

Inactive Publication Date: 2012-09-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide an equivalent and quantitative test method for the anti-white spot syndrome virus ability of prawns, so as to solve the problem that the current prawn disease resistance test process cannot be equal and quantitatively infected, and the food intake of prawns cannot be controlled. Accurately distinguish whether the prawns eat poisonous bait, whether the surviving prawns can be safely used for breeding and other unavoidable problems, so as to realize the accuracy and high efficiency of the WSSV resistance performance test of Chinese prawns, and overcome the shortcomings of the existing technology

Method used

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  • Equivalent and quantitative test method of white spot syndrome virus resistance of prawns

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 compares the prawn survival status of ingesting different levels of virus

[0030] The Chinese prawns used in the experiment were 320 larvae of "Huanghai No. 2" cultivated by the "China Penaeus Genetics and Breeding Center" in Aoshanwei, Qingdao City, Shandong Province in 2010, with a body length of 2-3cm. The breeding base is tested for artificial infection of WSSV, including 1), poison bait preparation; 2), anti-WSSV performance test; 3), data and sample collection; the specific method steps are as follows:

[0031] 1), poison bait preparation

[0032] (1) Detection of poisonous bait virus content

[0033] In order to test the disease resistance of prawns, it is first necessary to prepare poisonous baits with known virus content. Select infected prawns (preserved at -80 or just dead prawns) that have been killed by WSSV infection and have obvious symptoms of white spot syndrome, and use a scalpel to extract 5 mg of muscle tissue to extract genomic DNA. ...

Embodiment 2

[0044] The selection of the embodiment 2 reserved seed families and the detection of parental virus carrying capacity

[0045] The Chinese prawns used in the experiment are the "Huanghai No. 2" family bred by the "China Shrimp Genetics and Breeding Center" in Aoshanwei, Qingdao City, Shandong Province in 2011. There are a total of 104 families. Each family randomly selects 60 tails, with a body length of 2-3cm, and conducts VIE. (Visible implant elastomer) was temporarily raised after fluorescent labeling, and then transferred to Haiyang Huanghai Fishery Co., Ltd. Haizhenpin Breeding Base of Shandong Province after 2 days for artificial infection WSSV testing, including 1), preparation of poisonous bait; 2), anti-WSSV performance test; 3 ), data and sample collection; 4), determine the parent of the seed, the specific steps are as follows:

[0046] 1), poison bait preparation

[0047] (1) Detection of poisonous bait virus content

[0048] Method is with embodiment 1. The de...

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Abstract

An equivalent and quantitative test method of white spot syndrome virus resistance of prawns belongs to the technical field of disease-resistant prawn variety selection and includes: firstly, preparing poison bait; secondly, testing WSSV (white spot syndrome virus) resistance; thirdly, acquiring data and varieties; and fourthly, determining reserved parent seeds. The type of infecting virus is determined and the number of virus copies is detected by TaqMan real-time fluorescent quantitative PCR (polymerase chain reaction), the prawns having the same number of virus copies are used to prepare the poison bait, and the number of virus copies in muscular tissues in specific weight is calculated according to actual artificial infection needs. The equivalent and quantitative test method of the prawns is achieved by adding edible red pigment in the poison bait and infecting the prawns to be tested one by one, and obtaining true and reliable data is guaranteed.

Description

Technical field: [0001] The invention belongs to the technical field of selection and breeding of prawn disease-resistant varieties, and in particular relates to an equivalent and quantitative test method for the ability of prawns to resist white spot syndrome virus. Background technique: [0002] White Spot Syndrome virus (WSSV) was first discovered in Taiwan in 1992 (Chou HY, Huang CY, Wang CH, et al. Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan.Diseases of Aquatic Organisms, 1995, 23: 165~173), and the virus was quickly found in cultured prawns worldwide (Inouye K, Miwa S, Oseko N, et al.Mass mortalities of culture Kuruma shrimp Penaeus japonicus in Japan in 1993 .Fish Pathology.1994, 29:149~158 / Huang Jian, Yu Jia, Song Xiaoling, et al. Preliminary investigation on pathogen and transmission route of prawn outbreak in Zhejiang Province in 1994. Marine Fishery Research, 1995, 16(1): 91~98 / Jory D.E. & Dixon H.M. S...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12Q1/70C12Q1/68C12R1/93
CPCY02A40/81
Inventor 孟宪红张天时孔杰刘宁曹宝祥罗坤栾生
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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