Method for detecting toxicity of perfluorooctanoic acid in water by using wheel animalcule

A technology for perfluorooctanoic acid and water detection, applied in the field of environmental detection, can solve the problems of long test period, waste of resources, and difficulty in observing the influence of offspring's sexual characteristics.

Inactive Publication Date: 2012-09-12
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The biological growth cycle of these models and non-models is more than two weeks, the test cycle is long, it is not easy to observe its influence on the sexual characteristics of offspring, and due to the difference of various experimental conditions, there is a lack of comparability, which is a waste of resources

Method used

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  • Method for detecting toxicity of perfluorooctanoic acid in water by using wheel animalcule

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Experimental program
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Effect test

Embodiment 1

[0022] This embodiment relates to a method for detecting PFOA toxicity in water by using rotifers, including the following steps.

[0023] 1. Source and cultivation of rotifers

[0024] The tested rotifers were collected in Houhai, Beijing. The chemical indicators and molecular biological indicators were determined to be Brachionus calyxae. They were cultured at 20±1°C and under continuous light conditions (3000lx) for six months. The rotifer culture medium was The improved formula of rotifer culture solution (USEPA, 1985), the bait used is the freshwater green algae (C.pyrenoidosa) in the exponential growth stage cultured by the green algae medium, which is used after centrifugation and concentration, and the daily feeding density is 1.0× 10 6 cells mL -1 of green algae and replace the culture medium.

[0025] 2. Acute toxicity test

[0026] Perfluorooctanoic acid (PFOA) used for research was purchased from Sigma-Aldrich. Five concentrations were set up in the acute toxic...

Embodiment 2

[0033] Be that the PFOA solution of 0.5mg / L replaces the PFOA solution of 0.25mg / L in the step 3 in the embodiment 1 with concentration, other conditions are with embodiment 1; Experiment shows, compared with contrast, the PFOA of 0.25mg / L slightly Reduced rotifer population density.

Embodiment 3

[0035] Be that the PFOA solution of 1.0mg / L replaces the PFOA solution of 0.25mg / L in the step 3 in embodiment 1 with concentration, other conditions are with embodiment 1; Experiment shows that the PFOA of 1.0mg / L significantly reduces rotifer population Density poses a threat to the entire aquatic ecosystem.

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Abstract

The invention relates to a method for detecting biotoxicity of perfluorooctanoic acid (PFOA) in water by using wheel animalcule and belongs to the technical field of environment detection. Specifically, the wheel animalcule is taken as a model organism, the organism is wide in distribution, quick in breeding, and short in generation time, and the survival, the breeding, the behavior and the like are sensitive to the change of the environment physical and chemical factors; and the influence on the ecological system can be evaluated according to the change of the wheel animalcule population population density due to PFOA water solutions with different concentrations. According to the method, the difficulty that the existing method for detecting the PFOA biotoxicity in the water is long in period, and complex to operate, the convenient, sensitive and systematic method for detecting the PFOA toxicity in the water can be provided, and the method is convenient, quick and low in cost.

Description

technical field [0001] The invention relates to a method for detecting the toxicity of perfluorooctanoic acid in water by using Brachionus calyxae, and belongs to the technical field of environmental detection. Background technique [0002] Perfluorooctanoic acid (PFOA) is a perfluorinated organic acid with hydrophobic and oleophobic properties. It can make fluorides have extremely high surface activity on the surface of carbon oxides, and is resistant to high temperatures and strong oxidants. Its chemical structure is far superior to other surfaces. The active agent is stable, so it is widely used in hundreds of chemical industry, mechanical industry and daily perfluorinated chemical products. Since the 1950s, it has been widely used in the production of lint and carpet antifouling agents, mechanical lubricants, coatings, refrigerants, surface treatment agents, foam fire extinguishing agents, pharmaceuticals and cosmetics, as well as the production of electronic products an...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 牛军峰张利兰沈珍瑶
Owner BEIJING NORMAL UNIVERSITY
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