Method for detecting toxicity of PFOS (perfluorooctane sulfonate) in water through rotifers

A technology for perfluorooctane sulfonic acid and water detection, which is applied in the field of environmental detection and can solve the problems of rotifer toxicity that have not been reported.

Inactive Publication Date: 2012-09-26
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the aquatic ecotoxicology research of rotifers mainly involves heavy metal ion compounds, estrogens, polychlorinated biphenyls, polycyclic aromatic hydrocarbons, and pesticide pollutants, but the toxicity of PFOS to rotifers has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This embodiment relates to a method for detecting the toxicity of PFOS in water by using Brachionus calyciflora, including the following steps, wherein the concentration of PFOS in step 4 is 0.01 mg / L.

[0022] 1. Source and cultivation of rotifers

[0023] First, collect the test Brachionus calyxae rotifers, and use the rotifer culture medium to "clone" it in the laboratory, and use the freshwater green algae (C. , and the culture time is more than six months. Before the experiment, the rotifers were pre-cultured for more than two weeks with the rotifer culture liquid of the improved rotifer culture liquid formula (USEPA, 1985), and the daily feeding density was 1.0× during the pre-culture. 10 6 cells mL -1 The green algae were cultivated at 20±1°C under continuous light conditions (3000lx).

[0024] 2. Acute toxicity test

[0025] Perfluorooctanesulfonic acid (PFOS) was purchased from Sigma-Aldrich, and (PFOS) was set to 5 concentration gradients of 100.0, 10.0, 1...

Embodiment 2

[0031] Replace the PFOS solution of 0.01 mg / L in Step 4 in Example 1 with a PFOS solution with a concentration of 0.1 mg / L, and other conditions are the same as in Example 1. Experiments have shown that 0.1mg / L PFOS treatment prolongs the rotifer larval stage duration, shortens the average lifespan of rotifers, reduces the percentage of mixed females in the population, and then affects the ratio of males and females in the next generation of populations, and has no effect on the amount of eggs laid. obvious impact.

Embodiment 3

[0033]The PFOS solution with a concentration of 1.0 mg / L is used to replace the 0.01 mg / L PFOS solution in Step 4 of Example 1, and other conditions are the same as in Example 1. Experiments show that 1.0mg / L PFOS treatment inhibits the growth of rotifers, prolongs the duration of rotifer larval stage, shortens the average lifespan of rotifers, reduces the percentage of mixed females in the population, and then affects the male and female in the next generation population. ratio, and greatly inhibited the amount of eggs laid by rotifers.

[0034] Embodiment 1,2,3 experimental results are as shown in table 1:

[0035] Table 1. The larval stage time (T), average lifespan (ML) and

[0036] The average egg production (EQ) and the ratio of the number of mixed females / non-mixed females (MF / AF).

[0037] .

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PUM

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Abstract

The invention relates to a method for detecting biotoxicity of PFOS (perfluorooctane sulfonate) in water through rotifers, and belongs to the technical field of environmental detection. Particularly, the technical scheme comprises the steps that rotifers are selected as mode creatures, which are widely spread, quickly propagate, and have short generation time, and survival, propagation, activities and the like are sensitive to the variation of environment physical and chemical factors; the influence of the PFOS on the ecosystem can be evaluated through the change of the population density of rotifers caused by PFOS water solutions of different concentrations; and then the action mechanism of the PFOS can be confirmed through the influence of the PFOS on the life history of rotifers. The method overcomes the difficulties that the period is long and the operation is complicated during the biotoxicity detection for PFOS in water in the prior art, and provides the simple, convenient, sensitive and systemic method for detecting toxicity of PFOS in water; and the method is convenient, quick and low in cost.

Description

technical field [0001] The invention relates to a method for detecting the toxicity of perfluorooctane sulfonic acid in water by using rotifers, and belongs to the technical field of environmental detection. Background technique [0002] Due to its very unique physical and chemical properties such as hydrophobic and oleophobic properties, perfluorooctane sulfonic acid (PFOS) is widely used in industrial, commercial and personal consumer products, such as hydrophobic and oleophobic antifouling agents (carpets, textiles, interior decoration, leather, paper products, etc.), flame retardant (aerospace, fire protection), surfactant (fire extinguishing foam, alkaline cleaner), photoresist (semiconductor industry), electroplating anti-fog agent, Photo paper antistatic agent, paint additive, etc., and can be used as insecticide, herbicide, lubricant, adhesive and cosmetic ingredient. Currently, PFOS has been detected in varying concentrations in wastewater and sludge, surface water...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 牛军峰张利兰孙栋
Owner BEIJING NORMAL UNIVERSITY
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