Application of scolopendra mutilans neurotoxin peptide omega-SLPTX-Ssmla

A technology with fewer spiny centipedes and toxins, applied in the field of biomedicine, to achieve the effect of enhancing the current of calcium ion channels

Inactive Publication Date: 2012-09-19
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there has never been a report of polypeptide toxin as an activator of calcium ion channel

Method used

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  • Application of scolopendra mutilans neurotoxin peptide omega-SLPTX-Ssmla
  • Application of scolopendra mutilans neurotoxin peptide omega-SLPTX-Ssmla
  • Application of scolopendra mutilans neurotoxin peptide omega-SLPTX-Ssmla

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Embodiment Construction

[0017] 1. Separation and purification of omega-SLPTX-Ssm1a

[0018] The separation and purification of omega-SLPTX-Ssm1a is divided into three steps: (1) Molecular sieves; 4mL of centipede venom (absorption value at 280 nm is 200 OD) using a self-packed column (Sephadex G-50, 2.6 × 100 cm) separation. Use 0.1 M PBS (PH=6.0) for elution at a flow rate of 0.3 mL / min, and change the collection tube every ten minutes. The collected solution was detected by an ultraviolet spectrophotometer at the absorbance of each tube solution at 280 nm, and the elution curve was drawn. (2) Find out the elution peak where the target polypeptide is located, and further use reverse HPLC to separate and purify. The eluted peak of the molecular sieve was lyophilized and redissolved in 2ml of deionized water, and separated by a Phenomenex C18 (250 x 4.6 mm) chromatographic column. The elution solution was acetonitrile (containing 0.1% TFA), the flow rate was 3ml / min, and the detection wavelength wa...

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Abstract

The invention relates to application of scolopendra mutilans neurotoxin peptide omega-SLPTX-Ssmla, belonging to the field of biomedicine. A primary sequence of the omega-SLPTX-Ssmla comprises 83 amino acid residues, wherein three pairs of disulfide bonds are formed by six aminothiopropionic acids, and the molecular weight is 8811.3 Da. The code of the neurotoxin gene comprises 426 nucleotides of which include signal peptides, premise peptides and mature peptides. The scolopendra mutilans neurotoxin omega-SLPTX-Ssml has a quite obvious enhancement effect on calcium ion channel current on a dorsal ganglion root and is a novel calcium channel agonist. The scolopendra mutilans neurotoxin omega-SLPTX-Ssm provided by the invention can be used for increasing the openness of the calcium channel to enable calcium ions to flow internally and preparing medicaments for treating calcium channel diseases and can also be used as a new tool reagent for calcium channel researches.

Description

Technical field: [0001] The invention relates to the application of omega-SLPTX-Ssm1a, a polypeptide toxin of the less spiny centipede, and belongs to the technical field of biomedicine. Background technique: [0002] Calcium ion channels are an important class of transmembrane proteins on the cell membrane, not only regulating Ca 2+ The speed and amount of transmembrane movement, and participate in a series of Ca 2+ Rely on physiological activities, including muscle contraction, release of hormones and neurotransmitters, and intracellular signal transduction, etc. The Cav1 family is related to skeletal and smooth muscle contraction and neuroendocrine; Cav2 plays a very important role in the release of neurotransmitters and the control of neuron excitation. Calcium ion channels are composed of some auxiliary subunits such as α subunit and β subunit. The α subunit consists of four identical structural domains, and each domain contains six transmembrane segments. Although c...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P21/00A61P5/00A61P25/00A61P19/00
Inventor 赖仞杨仕隆容明强
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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