Construction method for rat model of increased abdominal pressure

Abstract

The invention relates to a construction method of animal models, which comprises the following steps of: 1, preparing an experimental animal; 2, carrying out anesthesia and incision; 3, carrying out intubation and connecting with an infusion and detection device; 4, making narrow portal vein backbone constant; 5, sewing up the incision and putting an abdominal binding belt; 6, exsanguinating and decompressing till shock; 7, recovering after shock; and 8, constructing a model successfully. The invention includes a series of correlative factors of abdominal hypertension occurrence and development, such as hemorrhagic shock, fluid resuscitation in quantity, and damage control operation, which are in accord with the course of disease in the clinical practice, provides a stable and reliable construction method which accords with the clinical practice for studying the abdominal hypertension, and thus the experimental study data obtained by using the method is more objective and accurate; the construction method can be widely used for studying the pathogenesis of the abdominal hypertension, the physiopathologic change of the body, the treatment effectiveness evaluation and the like.

Description

Technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing a rat model with elevated abdominal pressure. Background technique [0002] Abdominal hypertension is a state of elevated abdominal pressure caused by various reasons, and a clinical syndrome in which multiple organs and multiple systems such as heart, lung, brain, gastrointestinal, kidney, etc. change pathophysiologically. After investigation and research, Malbrain et al. found that as many as 32.1% of patients admitted to the intensive care ward had abdominal hypertension; and the mortality rate of patients caused by abdominal hypertension in the intensive care ward reached 30%-50%. [0003] The construction of a reasonable animal model is the basis for in-depth study of abdominal hypertension. In the past, several animal model construction methods have been developed, and most of these construction methods adopt a certain liquid (such as sodium la...

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Problems solved by technology

However, this method is carried out using pigs. There are disadvantages such as large experimental animals, inconvenient operation, a surgical team is required to complete the experiment, and the experiment costs a lot. It is not suitable for extensive application research.

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