Construction method for rat model of increased abdominal pressure

A technology of rat model and construction method, which is applied in the fields of medical science, active ingredients of alkali/alkaline earth metal chlorides, veterinary surgery, etc., and can solve problems such as unsuitable for extensive application research, large size of experimental animals, and high cost of experiments. To achieve the effect of convenient acquisition, low experiment consumption and low cost

Inactive Publication Date: 2012-10-03
常明涛 +1
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Problems solved by technology

However, this method is carried out using pigs. There are disadvantages such as large experimental animals, inconvenient operation, a sur

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  • Construction method for rat model of increased abdominal pressure
  • Construction method for rat model of increased abdominal pressure
  • Construction method for rat model of increased abdominal pressure

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Embodiment Construction

[0033] The present invention will be further explained below in conjunction with the drawings.

[0034] The method for constructing a rat model with elevated abdominal pressure according to the present invention has the following steps:

[0035] The first step is to prepare experimental animals: select 8 SD rats, both male and female, with a weight of 230-240g per animal. The animals are fasted for 12 hours before the experiment and freely drink water;

[0036] The second step, anesthetize and incision: Inject a 1.5% pentobarbital sodium solution into the abdominal cavity of the experimental animal—SD rat 1 at a dose of 30 mg / kg to anesthetize SD rat 1; SD rat Lie on the back after anesthesia, and fix the limbs on the small animal operating table; make a 3cm left inguinal incision 3 and a right inguinal incision 2 on the bilateral groin of SD rats, look for and bluntly separate the left thigh Artery 5, left femoral vein 7 and right femoral artery 4, right femoral vein 6, see figure ...

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Abstract

The invention relates to a construction method of animal models, which comprises the following steps of: 1, preparing an experimental animal; 2, carrying out anesthesia and incision; 3, carrying out intubation and connecting with an infusion and detection device; 4, making narrow portal vein backbone constant; 5, sewing up the incision and putting an abdominal binding belt; 6, exsanguinating and decompressing till shock; 7, recovering after shock; and 8, constructing a model successfully. The invention includes a series of correlative factors of abdominal hypertension occurrence and development, such as hemorrhagic shock, fluid resuscitation in quantity, and damage control operation, which are in accord with the course of disease in the clinical practice, provides a stable and reliable construction method which accords with the clinical practice for studying the abdominal hypertension, and thus the experimental study data obtained by using the method is more objective and accurate; the construction method can be widely used for studying the pathogenesis of the abdominal hypertension, the physiopathologic change of the body, the treatment effectiveness evaluation and the like.

Description

Technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing a rat model with elevated abdominal pressure. Background technique [0002] Abdominal hypertension is a state of elevated abdominal pressure caused by various reasons, and a clinical syndrome in which multiple organs and multiple systems such as heart, lung, brain, gastrointestinal, kidney, etc. change pathophysiologically. After investigation and research, Malbrain et al. found that as many as 32.1% of patients admitted to the intensive care ward had abdominal hypertension; and the mortality rate of patients caused by abdominal hypertension in the intensive care ward reached 30%-50%. [0003] The construction of a reasonable animal model is the basis for in-depth study of abdominal hypertension. In the past, several animal model construction methods have been developed, and most of these construction methods adopt a certain liquid (such as sodium la...

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Application Information

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IPC IPC(8): A61D1/00A61K33/14A61P43/00A61K31/19
Inventor 常明涛张连阳
Owner 常明涛
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