Urease-producing microorganisms and method for solidifying heavy metals in foundation using same

A microbial and urease-producing technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of incomplete removal, high cost, and generation of toxic sludge or other wastes, achieving good results and low cost Low, short-term effect

Active Publication Date: 2012-10-03
TSINGHUA UNIV
View PDF2 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional heavy metal treatment methods mainly include: chemical precipitation method, ion exchange method, evaporation concentration method, electrolysis method, activated carbon

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same
  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same
  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Isolation and Screening of Urease-Producing Bacteria with the Ability to Immobilize Heavy Metals

[0033] 1. Sample collection

[0034] Sporosarcina pasteurii was obtained from the American type culture collection (American type culture collection), numbered ATCC11859, and Terrabacter tumescens was obtained from the General Microbiology Center of China Committee for the Collection of Microbial Cultures, numbered AS.1.2690.

[0035] 2. Isolation and screening of urease-producing strains

[0036] Urease-producing strains were isolated from soil samples collected from the Tsinghua University flower garden.

[0037] Bacteria have ureolytic enzymes, which can decompose urea to produce a large amount of ammonia, making the medium alkaline and red. In this experiment, this characteristic was used to enrich and culture the test samples under the conditions of 37°C and 5M high-concentration urea for 24 hours, and kill various microbial vegetative cells that could not tolerate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Urease-producing microorganisms of Sporosarcina antarctica UR53, Sporosarcina koreensis UR47, Sporosarcina sp. UR31 and Bacillus lentus UR41 were preserved in China General Microbiological Culture Collection Center (CGMCC) on March 16, 2012 with the preservation numbers of CGMCC NO.5916, CGMCC NO.5915, CGMCC NO.5913 and CGMCC NO.5914, respectively. The method for solidifying heavy metals in the foundation by using the urease-producing microorganisms comprises the steps of: carrying out fermentation culture on the four microorganisms and microorganisms of Sporosarcina pasteuril and Terrabacter tumescens in a fermentation culture medium to obtain microorganism liquid, adding reaction liquid urea to the microorganism liquid, and then adding to the solution containing heavy metals to form a mixed solution, so that microorganism-heavy metal flocculates are formed in the mixed solution and then water-insoluble heavy metal carbonates are further formed. The method for solidifying the heavy metals in the foundation by using the microorganisms has the advantages of short solidifying time, good effect, low cost and no secondary pollution to environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and solidifying heavy metals thereof, in particular to urease-producing microorganisms and a method for solidifying heavy metals in foundations. Background technique [0002] Heavy metal pollution means that due to human production and activities, the content of heavy metals in the environment is significantly higher than its background value. Due to the long-term retention and non-degradable characteristics of heavy metal ions, it has caused great damage to the ecological environment. With large-scale urban expansion and construction, many buildings are built near abandoned factories and garbage dumps, and the heavy metal pollution in the foundation is serious. Heavy metal elements can finally enter the organism through the food chain, destroy the normal metabolism of the organism, and seriously endanger human health. It has become an environmental problem that cannot be ignored. [0003]...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/07
Inventor 程晓辉李萌郭红仙
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap