Chromogenic culture medium for separating and detecting shigella

A chromogenic medium, Shigella technology, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the problem of not being able to distinguish between Salmonella and Shigella, and difficult to detect Shigella at the same time Bacteria and black centers are not obvious, to reduce false positive and false negative results, simple result judgment, and less colony color

Active Publication Date: 2014-01-08
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, this medium cannot distinguish between Salmonella and Shigella; on the other hand, other bacteria that do not produce β-galactosidase will not be able to distinguish from Shigella, resulting in false positives
Although there are currently Shigella chromogenic media that use Salmonella to produce hydrogen sulfide to show black hearts on the medium to distinguish Salmonella, but Salmonella that do not produce hydrogen sulfide can still not be distinguished from Shigella, and some produce hydrogen sulfide Salmonella in the culture medium produces black centers that are not obvious and can also cause false positive results
[0005] To sum up, the current technical bottleneck of the medium used for isolation and detection of Shigella is: on the one hand, it is difficult to detect four species of Shigella at the same time in one medium, resulting in missed detection; On the one hand, it is difficult to distinguish Shigella from Enterobacteriaceae such as Salmonella, resulting in false positive results

Method used

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  • Chromogenic culture medium for separating and detecting shigella
  • Chromogenic culture medium for separating and detecting shigella
  • Chromogenic culture medium for separating and detecting shigella

Examples

Experimental program
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Embodiment 1

[0036]A color rendering medium for detecting and separating Zhigaobacteria, every 1000 ml medium contains yeast powder 4 g, aspirin 胨 3 g, soy protein 胨 6 g, sodium chloride 5 g, lactose 5 g, sucrose 5, sucrose 5, sucrose 5, sucrose 5, sucrose 5G, 2-deoxy-D-nucle sugar 5 g, agar powder 15 g, 5-bromo-4-chlorine-3-β-β-glucoside 0.4 g, 5-bromine-4-chlorine-3-吲哚-β-pyrarotidoside 0.2 g, 5-bromo-4-chlorine-3-吲哚 -n-acetyl-β-stalactitin 0.4 g, phenol red 0.08 g, No. 3 gallin 6 g, neonomycinSodium 4 mg, cephalosporine 9 mg, cephalosporin 肟 0.03 mg, the remaining amount is to remove ionic water, and the pH is 6.8 ± 0.2.

Embodiment 2

[0038] A color rendering medium for detecting and separating Zhigaobacteria, every 1000 ML medium contains yeast powder 6 g, aspotin 胨 3 g, soy protein 胨 7 g, sodium chloride 5 g, lactose 3 g, sucrose 8, 8 sucrose 8, sucrose 8G, 2-deoxy-D-nucle sugar 7 g, agar powder 18 g, 5-bromo-4-chlorine-3-β-β-glucoside 0.2 g, 5-bromine-4-chlorine-3- 吲哚-β-pyrarotidoside 0.1 g, 5-bromo-4-chlorine-3-吲哚 -n-acetyl-β-stalactitin 0.2 g, phenol red 0.06 g, No. 3 gallinin 5 g, neonomycinSodium 3 mg, cephalosporine 3 mg, cephalosporin 肟 0.05 mg, the remaining amount is to remove ionic water, and the pH is 6.8 ± 0.2.

Embodiment 3

[0040] A color rendering medium for detecting and separating Zhigaobacteria, every 1000 mL medium contains yeast powder 8 g, isproof 胨 2 g, soy protein 胨 6 g, sodium chloride 5 g, lactose 5 g, sucrose 5, sucrose 5, sucrose 5, sucrose 5, sucrose 5G, 2-deoxy-D-nucle sugar 10 g, agar powder 15 g, 5-bromo-4-chlorine-3-β-β-glucoside 0.1 g, 5-bromine-4-chlorine-3-吲哚-β-pyrarotidoside 0.2 g, 5-bromo-4-chlorine-3-吲哚 -n-acetyl-β-stalactitin 0.4 g, phenol red 0.04 g, No. 3 bile salt 7 g, neonomycinSodium 2 mg, cephalosporine 8 mg, cephalosporin 肟 0.07 mg, the remaining amount is to remove ionic water, and the pH is 6.8 ± 0.2.

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Abstract

The invention discloses a chromogenic culture medium for separating and detecting shigella; the culture medium comprises yeast powder, tryptone, soy peptone, sodium chloride, lactose, cane sugar, 2-deoxy-D-ribose, agar powder, beta-glucosaccharase chromogenic substrate, beta-pyranfucosidase chromogenic substrate, beta-galactosidase chromogenic substrate, phenol red, 3# cholate, novobiocin sodium salt, cefsulodin and cefixime; residue is water; the chromogenic culture medium provided by the invention can be used for separating and detecting four species of shigella and has advantages of high specificity, high sensitivity, easy operation, simple result judgment and the like; the chromogenic culture medium is suitable for detecting each sample and has wide application prospect; and detecting effect of the chromogenic culture medium can achieve level of like products in an import R&F company and is superior to inland like products.

Description

Technical field [0001] The invention involves a medium for microbial testing, especially a color rendering medium for separation and detection of Zhigee. Background technique [0002] Shigella (Shigella) belongs to the Department of Enterobacter, and there are four species, which are Diandu Zhiche ( Shigella dysenteriae ). Shigellaflexneri ), Bao Zhigaji ( Shigellaboydii ), Song Nei Zhigaji ( Shigellasonnei To.The four species of the genus Kaga can produce ulcerative colon infections of bacterial dysentery in the human body, and are the most important pathogen bacteria in bacterial dysentery.The pathogenic bacteria are generally transmitted through the food or water contaminated by excretion.Each year, a large number of reports caused by the infection of Zhigajee in the world, and the threat of the bacteria is increasingly threatened.Therefore, how to be separated from samples such as a large number of other bacteria containing a large number of other bacteria, which is separated...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/01
Inventor 黄岭芳邱国建卢勉飞蔡芷荷吴清平田亮
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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