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Method for separating and purifying ginsenoside Rb3

A technology of ginsenosides and total saponins, applied in organic chemistry, steroids, metabolic diseases, etc., can solve the problems of complex process and low yield, and achieve the effect of easy operation, high yield and no drug dependence

Active Publication Date: 2014-07-16
北京中云信合科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The general process of the patented technology for the separation and preparation of ginsenosides above is relatively complicated, and the yield is low, and there is no relevant information about ginsenoside Rb 3 Report on the purification method

Method used

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  • Method for separating and purifying ginsenoside Rb3
  • Method for separating and purifying ginsenoside Rb3
  • Method for separating and purifying ginsenoside Rb3

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Effect test

Embodiment 1

[0044] The object of the present invention is to provide a kind of active ingredient that extracts a kind of hypoglycemic effect from the total saponins of ginseng stems and leaves - ginsenoside Rb 3 extraction method. Specific steps are as follows:

[0045] (1) Preparation of sample glue

[0046] Accurately weigh 3.167g of total saponins from ginseng stems and leaves, dissolve them completely with 20mL of methanol, then add 6.447g of 80-100 mesh silica gel (the amount used is about twice the mass of total saponins), evaporate to dryness in a water bath at 50°C while stirring continuously, Do not agglomerate until the silica gel is in a dry powder state, and then dry it in a drying dish to make it a sample glue.

[0047] (2) Preparation of separation gel

[0048] Weigh 60.641g of 300-400 mesh silica gel (the amount used is about 20 times the mass of total saponins), fully dissolve it with 200mL of dichloromethane, and ultrasonically remove air bubbles for 20 minutes while ...

Embodiment 2

[0058] (1) Purity test

[0059] The ginsenoside Rb purified by column chromatography was purified by high performance liquid chromatography (HPLC). 3 Detected, its purity is 90.17%. Chromatographic conditions: column, -C 18 (3.9mm×150mm, 4μm); mobile phase, methanol:water:phosphoric acid volume ratio is 65:35:1; flow rate, 1.0mL / min; detection wavelength, 203nm; injection volume, 20μL. Under this chromatographic condition, ginsenoside Rb 3 The regression equation of the standard curve is y=445193.3113x-175865.1971, R=0.9999; the injection volume has a good linear relationship in the range of 0.8μg~20μg.

[0060] (2) Ginsenoside Rb 3 structural analysis of

[0061] The structure of the purified product was characterized by infrared absorption spectroscopy and nuclear magnetic resonance spectroscopy, and ginsenoside Rb was obtained. 3 IR and NMR spectral features. The instruments selected were Nicolet 550 Fourier transform infrared spectrometer (Nicolet company USA) and...

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Abstract

The invention relates to a method for extracting Chinese medicinal bioactive ingredients, in particular to a method for separating and purifying ginsenoside Rb3 serving as an active ingredient for reducing blood sugar. According to the method, total saponins of ginseng stems and leaves are used as a raw material and mixed with methanol and silica gel, and the mixture is prepared into powdery sample glue by water bath evaporation to dryness. By using dry loading, the problem that the total saponins of the ginseng stems and leaves are difficult to dissolve is solved. The high-purity ginsenoside Rb3 is prepared by adopting column chromatography separation and purification. The method is easy to operate, high in yield, low in cost and the like, and does not introduce toxic substances; and the ginsenoside Rb3 can be used for treating and preventing diabetes. The method has significance for research of preparation and development of the functional ginsenoside.

Description

technical field [0001] The invention belongs to the field of separation and purification methods of active components of traditional Chinese medicines, in particular to ginsenoside Rb 3 separation and purification methods. Background technique [0002] Diabetes is a major health problem facing modern humans. According to statistics, 3% of the world's population suffers from diabetes, and diabetes has been listed as one of the top ten "killers" in the world. Therefore, it is imminent to actively prevent and treat diabetes. Modern epidemiological studies have shown that hyperglycemia is the main cause of complications in diabetic patients. The key to preventing and resisting diabetic complications and improving the quality of life of diabetic patients is to effectively control blood sugar. Ginseng (Panax ginseng C.A.Mey.) is a plant of the genus Panax in the family Araliaceae, and is a precious medicinal material specially produced in my country. In "Shen Nong's Materia Me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00A61P3/10
Inventor 弓晓杰陈丽荣李珂珂马畅盖鑫
Owner 北京中云信合科技有限公司