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Ganoderma lucidum polysaccharide of high molecular weight and detection method thereof

A Ganoderma lucidum polysaccharide and high-molecular-weight technology, applied in material separation, analytical materials, measuring devices, etc., can solve the problems of unreported preparation methods, and achieve the effects of simple preparation methods, accurate detection methods, and improved immune activity

Inactive Publication Date: 2012-10-10
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this polysaccharide can be obtained through complex separation and purification methods in the laboratory, large-scale preparation methods have not been reported.

Method used

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  • Ganoderma lucidum polysaccharide of high molecular weight and detection method thereof
  • Ganoderma lucidum polysaccharide of high molecular weight and detection method thereof
  • Ganoderma lucidum polysaccharide of high molecular weight and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 high molecular weight ganoderma polysaccharide

[0029] 1. Water extraction and alcohol precipitation

[0030] Add 10 times of water to 50L of ganoderma lucidum fruiting body and heat at 100°C for 1 hour, filter, add 10 times of water to the residue and heat at 100°C for 1 hour, filter, combine filtrate 160L, concentrate to 5L with a rotary evaporator, add 5L of absolute ethanol to 50% concentration, let it stand overnight, and remove the supernatant after the pellet was centrifuged.

[0031] 2. Purification of Ganoderma lucidum polysaccharide

[0032] After the precipitate was dissolved in water, ultrafiltration was performed with an ultrafiltration membrane with an upper cut-off value of 500,000 to obtain 36 g of high molecular weight Ganoderma lucidum polysaccharide greater than 500,000.

[0033] 3. Further Purification and Concentration

[0034] Add 0.5 g of Ganoderma lucidum polysaccharides greater than 500,000 to a 50 mm×300 mm D...

Embodiment 2

[0037] Example 2 Bioactivity detection, the high molecular weight Ganoderma lucidum polysaccharide used was prepared in Example 1.

[0038] 1 Establishment of mouse H22 xenograft tumor model

[0039] Aseptic ascites was extracted from well-grown H22 ascites tumor mice (purchased from Nanjing University of Traditional Chinese Medicine), and diluted with normal saline to adjust the number of tumor cells to 3×10 7 / ml cell suspension, use 75% ethanol to sterilize the right axillary skin of experimental BALB / c mice (purchased from Shanghai Xipuer-Bikay Experimental Animal Co., Ltd.), and inject 0.2ml H22 ascites tumor subcutaneously into the right axillary of each mouse Cell suspension (containing tumor cells 6×10 6 ).

[0040] 2 grouping and dosing

[0041] After 24 hours of inoculation, 30 BALB / c mice were randomly divided into three groups for administration: the model group was given the same amount of normal saline, the 5-FU group was given 20 mg / kg, and the high molecular...

Embodiment 3

[0050] The mensuration of embodiment 3 high molecular weight ganoderma polysaccharides

[0051] 1. Preparation of Standards

[0052] 20 mg of high molecular weight Ganoderma lucidum polysaccharide was dissolved in water to form a 10 mg / mL solution, applied to a Sephacryl 500 gel chromatography column, the first sugar peak was collected, concentrated, and freeze-dried to obtain 15 mg of high molecular weight Ganoderma lucidum polysaccharide.

[0053] 2. Testing of Standards

[0054] HPLC detects, and the result shows that the standard substance is a single peak under the following liquid phase conditions (see figure 1 ).

[0055] Chromatographic column: TSKgel G6000PWXL(13) 7.8*300 (from Tosoh Bioscience); mobile phase: 0.05mol / L NaH2PO4 and 0.15mol / L NaNO3 aqueous solution; flow rate: 0.5mL / min; column temperature: 35°C; differential detector.

[0056] 3. Detection of samples to be tested

[0057] Accurately weigh 10mg of Ganoderma lucidum fruiting body sample, add 1000...

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Abstract

The invention provides ganoderma lucidum polysaccharide of high molecular weight and a detection method thereof. The ganoderma lucidum polysaccharide of high molecular weight is obtained via water extraction, alcohol precipitation, purification, and freeze-drying. The preparation method of the ganoderma lucidum polysaccharide of high molecular weight is simple and is suitable for large-scale production. The polysaccharide content is more than 65 percent, and macrophages can be stimulated to produce nitric oxide (NO).

Description

technical field [0001] The invention relates to the field of medicinal fungi, in particular to a high molecular weight ganoderma polysaccharide and a detection method thereof. Background technique [0002] Ganoderma lucidum polysaccharide is one of the main medicinal ingredients in the medicinal fungus Ganoderma lucidum, which has various biological activities such as immune regulation, anti-tumor, anti-aging, and anti-hypertension. Ganoderma lucidum contains a high-molecular-weight polysaccharide component of Ganoderma lucidum with a molecular weight of more than 1000kDa. This polysaccharide can selectively stimulate B cells and macrophages, significantly enhance the production of immunoglobulins, and enhance the phagocytosis of macrophages and stimulate macrophages. Phage cells produce the cytokines IL-1β and TNF-α production. Although this polysaccharide can be obtained through complex separation and purification methods in the laboratory, the large-scale preparation met...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/00A61P35/00G01N30/02
Inventor 唐庆九王晨光张劲松张圣龙周帅刘艳芳杨焱唐传红
Owner SHANGHAI ACAD OF AGRI SCI
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