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Inducer for inducing directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells, and preparation and application thereof

A technology of smooth muscle cells and mesenchymal stem cells, which is applied in the field of inducers to promote the directed differentiation of umbilical cord mesenchymal stem cells, and can solve problems such as the differentiation of mesenchymal stem cells into bladder smooth muscle cells.

Inactive Publication Date: 2012-10-17
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SDF-1 plays a key role in the homing of hematopoietic stem cells to the bone marrow during embryonic development and after stem cell transplantation. SDF-1 is also important in cardiogenesis and angiogenesis. In addition, SDF-1 can improve trauma in patients, especially diabetic patients Or ulcer healing, but there is no report of SDF-1 in vitro induction of mesenchymal stem cells into bladder smooth muscle cells, and there is no report of SDF-1 in vitro induction of human umbilical cord mesenchymal stem cells into bladder smooth muscle cells

Method used

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  • Inducer for inducing directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells, and preparation and application thereof
  • Inducer for inducing directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells, and preparation and application thereof
  • Inducer for inducing directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 In vitro induction of human umbilical cord mesenchymal stem cells

[0031] (1) Take stromal cell-derived factor-1;

[0032] (2) Umbilical cord mesenchymal stem cells were taken and subcultured in vitro. The culture medium was L-DMEM medium containing 10% fetal bovine serum, and stromal cell-derived factor-1 and stromal cell-derived factor-1 were added to the culture medium. The concentration was 25 μg / ml, and the culture was induced for 7 days.

Embodiment 2

[0033] Example 2 Induction of human umbilical cord mesenchymal stem cells in vitro

[0034] (1) Take stromal cell-derived factor-1;

[0035] (2) Umbilical cord mesenchymal stem cells were taken and subcultured in vitro. The culture medium was L-DMEM medium containing 10% fetal bovine serum, and stromal cell-derived factor-1 and stromal cell-derived factor-1 were added to the culture medium. The concentration was 50 μg / ml, and the culture was induced for 7 days.

Embodiment 3

[0036] Example 3 In vitro induction of human umbilical cord mesenchymal stem cells

[0037] (1) Take stromal cell-derived factor-1;

[0038] (2) Umbilical cord mesenchymal stem cells were taken and subcultured in vitro. The culture medium was L-DMEM medium containing 10% fetal bovine serum, and stromal cell-derived factor-1 and stromal cell-derived factor-1 were added to the culture medium. The concentration was 75 μg / ml, and the culture was induced for 7 days.

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Abstract

The invention discloses application of stroma cell-derived factor-1 in preparing an inducer used for inducing directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells. The invention also discloses the inducer used for inducing directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells, application of the inducer and a method for promoting directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells. The inducer provided in the invention can promote directional differentiation of umbilical cord mesenchymal stem cells into bladder smooth muscle cells and has a good application prospect and good economic benefits when used for repair of bladder injury.

Description

technical field [0001] The invention relates to an inducer for promoting directional differentiation of umbilical cord mesenchymal stem cells, in particular to an inducer for inducing human umbilical cord mesenchymal stem cells to differentiate into bladder smooth muscle cells in vitro. Background technique [0002] Various diseases of the bladder, such as: congenital deformities, acquired injuries, severe inflammatory infections, etc., will lead to the loss of its structure or function, which needs to be repaired and reconstructed. The traditional method is mainly to use the patient's own gastrointestinal tract tissue to perform bladder enlargement surgery. These methods have complications such as reduced neobladder capacity, metabolic disorders, intestinal obstruction, stone formation, perforation, and even malignant lesions, leading to bladder repair and reconstruction. Difficulties in the clinical work of urology. The rise of tissue engineering technology and its succes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N5/071
Inventor 韩平员海超魏强王坤杰魏鑫李虹
Owner WEST CHINA HOSPITAL SICHUAN UNIV